A novel solid-phase synthetic method for production of <i>N</i>-alkyl-4-alkylamino-1-aryl-1<i>H</i>-pyrazolo[3,4-<i>d</i>]pyrimidine-6-carboxamide library

다국어 초록 (Multilingual Abstract)

<P><B>Abstract</B></P> <P>This work describes a solid-phase synthetic method for building a compound library of <I>N</I>-alkyl-4-alkylamino-1-aryl-1<I>H</I>-pyrazolo[3,4-<I>d</I>]pyrimidine-6-carboxamide derivatives, that are based on the biologically active 1-aryl-1<I>H</I>-pyrazolo[3,4-<I>d</I>]pyrimidine scaffold. In the first step of this synthetic sequence, condensation reactions of ethyl 5-amino-1-aryl-1<I>H</I>-pyrazole-4-carboxylates with methyl cyanoformate resulted in the formation of esters that underwent hydrolysis to give 1-aryl-4,5-dihydro-1<I>H</I>-pyrazolo[3,4-<I>d</I>]pyrimidin-4-one-6-carboxylic acids. The coupling reaction of these carboxylic acids with primary alkylamine-loaded acid-sensitive methoxybenzaldehyde (AMEBA) resins was followed by amination reactions mediated by benzotriazol-1-yloxytris(dimethylamino)phosphonium hexafluorophosphate (BOP). Subsequent cleavage from the solid support resulted in the formation of the target <I>N</I>-alkyl-4-alkylamino-1-aryl-1<I>H</I>-pyrazolo[3,4-<I>d</I>]pyrimidine-6-carboxamide derivatives. The reaction conditions for solid-phase transformations were optimized using a solution-phase model study with 2,4-dimethoxybenzyl-protected isobutylamine as a reactant in place of the AMEBA resin-loaded isobutylamine. The progress of the solid-phase reactions was monitored by on-bead ATR-FTIR spectroscopy. Diversification experiments were performed by using 1-aryl-4,5-dihydro-1<I>H</I>-pyrazolo[3,4-<I>d</I>]pyrimidin-4-one-6-carboxylic acids and a variety of primary and secondary amine building blocks to build the target compound library.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>