High mobility group box-1 and inflammatory cytokines in follicular fluid of patient with Endometriosis = High mobility group box-1 and inflammatory cytokines in follicular fluid of patient with Endometriosis

국문 초록 (Abstract)

목적: The aim of the study was to examine the inflammatory status of follicular fluid in endometriosis by detecting High mobility group box-1 (HMGB-1) and inflammatory cytokines, which reflect the milieu of oocyte that may play a role for infertility.
방법: Total 60 patients who had received in vitro fertilization and embryo transfer (IVF-ET) from March, 2013 to March, 2016 were included: 30 patients who had endometriosis as case group, 30 participants without endometriosis as the control. Follicular fluid (FF) was obtained from a dominant follicle during oocyte retrieval and stored at -70°C. Level of HMGB-1 was measured with ELISA, as well as inflammatory cytokines including Interleukin (IL)-1β, IL-6, IL-8, and tumor-necrosis factor (TNF)-α.
결과: Level of HMGB-1 was significantly higher in FF samples from the endometriosis group compared to the controls. IL-6, IL-8, and TNF-α levels were significantly higher in the endometriosis group compared to those in the control. There were significant positive correlations among the four inflammatory cytokines. The levels of the inflammatory cytokines positively correlated with the levels of HMGB-1 in the FF samples. TNF-α levels were negatively correlated with the cumulative embryo score per embryo. Logistic regression analysis revealed that the number of high-quality embryos was an independent factor predicting clinical pregnancy.
결론: This study showed that HMGB-1 and inflammatory cytokines may play an important role through NF-kB pathway, in inflammatory cascade affecting oocyte milieu.