Anti-inflammatory and in vitro bone formation effects of Garcinia mangostana L. and propolis extracts

다국어 초록 (Multilingual Abstract)

The purpose of this study was to determine the anti-inflammatory and in vitro bone formation effects of Garcinia mangostana L. (mangosteen) and propolis extracts. Immortalized human gingival fibroblasts (hTERThNOF) cells were treated with Porphyromonas gingivalis KCOM 2804 lipopolysaccharide followed by treatment with mangosteen and propolis extract alone or in combination.
Expression levels of inflammatory cytokines were evaluated by enzyme-linked immunosorbent assay. Effect of mangosteen and/or propolis extracts on mineralization of MG-63 cells was evaluated by alkaline phosphatase activity and alizarin red S staining. Group mangosteen extract complex 1:34 (1 lg/ml mangosteen extract and 34 lg/ml propolis extract) significantly reduced expression levels of IL-6, IL-8, and PGE2. It had higher than other groups in vitro bone formation effect on MG63 cells. These results suggest that mangosteen and propolis extract complex could be used in the prevention and treatment of periodontal disease.

참고문헌 (Reference)

1 Johnson JJ, "a-Mangostin, a xanthone from mangosteen fruit, promotes cell cycle arrest in prostate cancer and decreases xenograft tumor growth" 33 : 413-419, 2012

2 Gutierrez-Orozco F, "a-Mangostin : anti-inflammatory activity and metabolism by human cells" 61 : 3891-3900, 2013

3 Palombo EA, "Traditional medicinal plant extracts and natural products with activity against oral bacteria : potential application in the prevention and treatment of oral diseases" 2011 : 1-15, 2009

4 Nakashima K, "The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation" 108 : 17-29, 2002

5 Komori T, "Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts" 89 : 755-764, 1997

6 Altan BA, "Systemic propolis stimulates new bone formation at the expanded suture : a histomorphometric study" 83 : 286-291, 2013

7 Socransky SS, "Subgingival microbial profiles in refractory periodontal disease" 29 : 260-268, 2002

8 Sa´nchez GA, "Salivary IL-1b and PGE2 as biomarkers of periodontal status, before and after periodontal treatment" 40 : 1112-1117, 2013

9 Ramos AFN, "Propolis : a review of its anti-inflammatory and healing actions" 13 : 697-710, 2007

10 Liu J, "Porphyromonas gingivalis promotes the cell cycle and inflammatory cytokine production in periodontal ligament fibroblasts" 60 : 1153-1161, 2015

11 Borrelli F, "Phytochemical compounds involved in the anti-inflammatory effect of propolis extract" 73 : 53-63, 2002

12 Puchtler H, "On the history and mechanism of alizarin and alizarin red S stains for calcium" 17 : 110-124, 1969

13 Suzuki N, "Mixed red-complex bacterial infection in periodontitis" 2013 : 587279-, 2013

14 Idrus E, "Mangosteen extract inhibits LPS-induced bone resorption by controlling osteoclast" 9 : 362-367, 2016

15 Zhao J, "Local and peripheral cytokines profiling on Porphyromonas gingivalis induced rat periodontitis model" 9 : 5996-6004, 2016

16 Okada N, "Interleukin-6 production in human fibroblasts derived from periodontal tissues is differentially regulated by cytokines and a glucocorticoid" 32 : 559-569, 1997

17 Raso GM, "Inhibition of inducible nitric oxide synthase and cyclooxygenase-2 expression by flavonoids in macrophage J774A. 1" 68 : 921-931, 2001

18 Michaluart P, "Inhibition effects of caffeic acid phenethyl ester on the activity and expression of cyclooxygenase-2 in human oral epithelial cells and in a rat model of inflammation" 59 : 2347-2352, 1999

19 van den Dolder J, "Flow perfusion culture of marrow stromal osteoblasts in titanium fiber mesh" 64 : 235-241, 2003

20 Heim KE, "Flavonoid antioxidants : chemistry, metabolism and structure-activity relationships" 13 : 572-584, 2002

21 Pai MR, "Evaluation of antiplaque activity of Azadirachta indica leaf extract gel—a 6-week clinical study" 90 : 99-103, 2004

22 Occhiuto F, "Effects of phytoestrogenic isoflavones from red clover (Trifolium pratense L.) on experimental osteoporosis" 21 : 130-134, 2007

23 Feng J, "Effects of metabolites of the lignans enterolactone and enterodiol on osteoblastic differentiation of MG-63 cells" 31 : 1067-1070, 2008

24 Tredwin C, "Drug-induced disorders of teeth" 84 : 596-602, 2005

25 Fujita Y, "Correlations between pentraxin 3 or cytokine levels in gingival crevicular fluid and clinical parameters of chronic periodontitis" 100 : 215-221, 2012

26 Kern B, "Cbfa1 contributes to the osteoblast-specific expression of type I collagen genes" 276 : 7101-7107, 2001

27 Reinholz GG, "Bisphosphonates directly regulate cell proliferation, differentiation, and gene expression in human osteoblasts" 60 : 6001-6007, 2000

28 Gutierrez-Orozco F, "Biological activities and bioavailability of mangosteen xanthones : a critical review of the current evidence" 5 : 3163-3183, 2013

29 Tjahjani S, "Antioxidant properties of Garcinia mangostana L. (Mangosteen) Rind" 13 : 198-203, 2014

30 Cushnie TP, "Antimicrobial activity of flavonoids" 26 : 343-356, 2005

31 Nguyen PT, "Antimicrobial actions of a-mangostin against oral streptococci" 57 : 217-225, 2011

32 Yiemwattana I, "Antibacterial and antiinflammatory potential of Morus alba stem extract" 12 : 265-274, 2018

33 Chen LG, "Anti-inflammatory activity of mangostins from Garcinia mangostana" 46 : 688-693, 2008

34 Yiemwattana I, "Alpha-mangostin suppresses IL-6and IL-8 expression in LPS-stimulated human gingival fibroblasts" 103 : 348-355, 2015