生物防除菌 Pseudomonas stutzeri YPL-1이 가지는 藥劑耐性 Plasmid의 Curing = Plasmid Curing of Antagonistic Pseudomonas stutzeri YpL-1 by Ethidium Bromide

다국어 초록 (Multilingual Abstract)

Pseudomonas stutzeri YPL-1 inhibited strongly the growth of root-rotting fungi fusarium solani was isolated from the Ginseng root-rot suppressive soil. Curing agents such as ethidium bromide, sodium dodecyl sulfate were used to cure the R -plasmid of antagonistic Pseudomonas stutzeri YPL-1 strain which have antibiotic resistance. Minimal growth inhibitory concentrations (MIC) of tetracyclin, ampicillin and streptomycin on the growth of Pseudomonas stutzeri YPL-1 were found to be>200,>100, and>100μg/ml, respectively. And so we found that the strain was susceptible to kanamycin, chloramphenicol, and gentamycin. The curing treatments of YPL-1 strain with ethidium bromide, sodium dodecyl sulfate resulted in the elimination of R-plasmid from the strain. In result, ethidium bromide was proved to be the most powerful curing agent for the elimination of R-plasmid in the strain and the curing frequency with ethidium bromide( 10μg / ml ) was about 81.7%, in case of sodium dodecyl sulfate(100 μg / ml ) was about 47%.Optimal pH for the elimination of R-plasmid was pH 7.5 and the R-plasmid in the cells incubated for 24hours with ethidium bromide was proved to be eliminated most effectively.