P272 : Inhibitory effects of polydeoxyribonucleotide on melanogenesis = P272 : Inhibitory effects of polydeoxyribonucleotide on melanogenesis

다국어 초록 (Multilingual Abstract)

Background: Polydeoxyribonucleotide (PDRN), extracted from the sperm of trout, contains deoxyribonucleotide polymers consisted of 50-2000 base pairs. It activates A2receptor and salvage pathway, which induces secretion of growth factors, vascular endothelial growth factor (VEGF) and anti-inflammatory cytokines. PDRN, mainly applied as injection, is widely used for treating various disorders such as diabetic foot ulcer, scars, fine wrinkles, alopecia, and vascular insufficiency. However, the effects of PDRN on melanogenesis are not well known. Objectives: To investigate the effect of PDRN on melanogenesis. Methods: We examined the effect of PDRN on melanogenesis using Mel ab mouse melanocyte cells and human melanocytesby measurement melanin contents and activity of tyrosinase after PDRN treatment. Microphthalmia-associated transcription factor (MITF) and tyrosinase protein were examined by Western blotting. The action mechanism of PDRN on melanogenesis by stimulation of A2 receptor was also investigated. Results: PDRN inhibited the activity of tyrosinase as well as level of melanin contents in dose dependent manner, which tended to decrease more sharply in tyrosinase activity. The levels of MITF and tyrosinase protein were also decreased after PDRN treatment. Conclusion: These results suggest that PDRN can be used as an anti-melanogenic agent.