보두산(寶豆散)메탄올 추출물의 항염증 효과 = Anti-inflammatory Effect of Bodusan

다국어 초록 (Multilingual Abstract)

Objectives: Inflammation is important event in the development of vascular diseases including hypertension, atherosclerosis, and restenosis. Bodusan (BDS) was a traditional Korean herbal medicine and widely used in treatment of gastrointestinal complaint and stomach ulcer. The aim of this study was to determine whether BDS and its components inhibit production of nitrite, PGE2 and proinflammatory cytokines in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages. Methods: Cytotoxic activity of BDS and its components on RAW 264.7 cells was using 5-(3-caroboxymcrophages.eth-oxyphenyl)-2H-tetra-zolium inner salt (MTS) assay. The nitric oxide (NO) production was measured by Griess reagent system. And proinflammatory cytokines were measured by ELISA kit. The levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression were detected by western blot. Results: Our results indicated that BDS and its components significantly inhibited the LPS-induced NO and PGE2 production. Moreover, BDS and its components inhibited iNOS and COX-2 expression accompanied by an attenuation of TNF-α, IL-1β, IL-6 and MCP-1 formation in macrophages. Conclusions: These results indicate that BDS and its components have potential as an anti-inflammatory agent.