Oleanolic acid acetate attenuates polyhexamethylene guanidine phosphate-induced pulmonary inflammation and fibrosis in mice

다국어 초록 (Multilingual Abstract)

<P><B>Abstract</B></P> <P>Oleanolic acid acetate (OAA), triterpenoid compound isolated from <I>Vigna angularis</I> (azuki bean), has been revealed anti-inflammatory in several studies. We investigated the effects of OAA against polyhexamethylene guanidine phosphate (PHMG-P)-induced pulmonary inflammation and fibrosis in mice. OAA treatment effectively alleviated PHMG-P-induced lung injury, including the number of total and differential cell in BAL fluid, histopathological lesions and hydroxyproline content in a dose dependent manner. Moreover, OAA treatment significantly decreased the elevations of IL-1β, IL-6, TNF-α, TGF-β1, and fibronectin, and the activation of the NOD-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome in the lungs of PHMG-P-treated mice. Cytokines are known to be key modulators in the inflammatory responses that drive progression of fibrosis in injured tissues. The activation of NLRP3 inflammasome has been reported to be involved in induction of inflammatory cytokines. These results indicate that OAA may mitigate the inflammatory response and development of pulmonary fibrosis in the lungs of mice treated with PHMG-P.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We investigated the effects of anti-inflammation and anti-fibrosis by intratracheal instillation of OAA in PHMG-P-induced pulmonary injury. </LI> <LI> OAA attenuated PHMG-P-induced pulmonary injury, including the number of total and differential cell in BAL fluid and histopathological changes. </LI> <LI> OAA reduced the activation of NLRP3 inflammasome as well as the expression of pro-inflammatory and pro-fibrotic cytokines induced by PHMG-P. </LI> </UL> </P>