PSEUDOMONAS AERUGINOSA(錄膿菌)에 關한 實驗的 硏究 = Experimental Studies on Pseudomonas aeruginosa

다국어 초록 (Multilingual Abstract)

Since the original report of Gessard in 1882, Ps. aeruginosa has been known as blue pus-producing becteria. Being produced by the organisms during the growth and multiplication, one of the pigments, pyocyanin, was discovered by Emmerich and Loew and confirmed later to possess an antibiotic activities to most Gram positive cocci. The fact seemed to be incompatible with the clinical findings that there are many cases of mixed infections due to pseudomonas and Gram positive cocci. It was assumed that the physiology of pseudomonas, including the pigment productions, might be different in vivo conditions in the inflammatory lesions from in vitro culture conditions. Though the present experimental technology would not make possible the exact duplication of in vivo conditions in vitro, it was thought to be worth-while to observe the bacterial behaviors under varied cultural conditions.
Thus 20 strains of Ps. aerguinosa were cultured at 30℃ and 37℃ aerobically and at 40℃ under decreased O_2 and incrcreased CO_2 tensions in candle-jar, which is said to be nearer to the conditions in most of closed inflammatory lesions (Dubos). Some biological features of the organisms cultivated under varied conditions were studied and viable organisms of the cultures, pseudomonas alone or mixed with the suspension of Staph. aureus culture, were inoculated subcutaneously into the abdominal regions of rabbits.
Results of the studies might be summarized as follows:
1.Compared to the organisms cultured below 37℃, the cells of Ps. aerguinosa, when they were grown above 37℃., were larger and motility more sluggish.
2.Of the increase of viable units in nutrient broth, no significant difference was noticed among the cultures grown under the varied conditions.
3.Compared to the aerobic growth at 30℃. or 37℃., the pigment productions of Ps. aeruginosa strains were noticiably diminished at 40℃. in candle-jar.
4.Biochemically the pseudomonas strains appeared to be more active at 40℃. in candle-jar than under the other cultural conditions studied.