Isoproterenol과 Melatonin의 첨가가 돼지 단위발생란의 체외발달에 미치는 영향

다국어 초록 (Multilingual Abstract)

In this study, to improve the in vitro development of various cells including cloned embryos, the effects that isoproterenol and melatonin have on in vitro development of porcine parthenogenetic oocytes were investigated. Parthenogenetic activation was induced with electrical stimulation, BSA and 6-DMAP treatment. 10-7 M of melatonin and isoproterenol (10-10, 10-12 and 10-14 M) were supplemented for in vitro maturation (IVM) and in vitro culture (IVC) medium, with different concentrations. When isoproterenol and melatonin were supplemented in IVM medium with different concentrations, there was no significant (P<0.05) difference of maturation rate in the treatment groups as well as in that of only melatonin. As isoproterenol and melatonin were supplemented in IVM medium with different concentrations, blastocyst rates of isoproterenol 10-12 M treatment group (37.1%) were significantly (P<0.05) higher than control group (26.0%). Isoproterenol and melatonin were supplemented in IVC medium with different concentrations, then the cleavage rate of 10-12 M isoproterenol treatment group (82.2%) was significantly (P<0.05) higher than the group that melatonin was only supplemented (70.9%). There was no difference of blastocyst rate between the treatment groups. When isoproterenol and melatonin were supplemented for IVM+IVC medium with different concentrations, the cleavage rate of 10-12 M isoproterenol treatment group (92.5%) was significantly (P<0.05) higher than the control group (82.8%) and the group that melatonin was only treated (81.6%). The blastocyst rate of 10-12 M as 45.6% was significantly (P<0.05) higher than control group (25.2%) and melatonin treatment group (31.2%). The cell number of blastocyst in 10-12 M isoproterenol treatment group 35.5±3.4 was significantly (P<0.05) highest. The results of this study showed that the development rate of IVC when both isoproterenol and melatonin were supplemented was higher than when melatonin was only supplemented. Therefore, it is concluded that isoproterenol is rather effective in the activation of melatonin. 10-7 M melatonin and 10-12 M isoproterenol were considered suitable concentration.

목차 (Table of Contents)

• 서 론
• 재료 및 방법
• 1. 난포란의 채란 및 체외 성숙
• 2. 성숙 난자의 단위 발생 유기
• 3. 단위발생란의 체외 배양 및 핵 수 조사
• 4. 실험 설계
• 5. 통계학적 분석
• 결 과
• 1. 돼지 난포란의 체외 성숙에 있어서 Isoproterenol과 Melatonin의 첨가 농도에 따른 체외 성숙율
• 2. 돼지 난포란의 체외 성숙에 있어서 Isoproterenol과 Melatonin의 첨가 농도에 따른 단위발생란의 체외 발달율
• 3. 돼지 단위발생란의 체외 배양에 있어서 Isoproterenol과 Melatonin의첨가 농도에 따른 체외 발달율
• 4. 돼지 난포란의 체외 성숙과 배양시 Isoproterenol과 Melatonin의 첨가 농도에 따른 단위발생란의 체외 발달율
• 고 찰
• 결 론
• REFERENCES

참고문헌 (Reference)

1 정다운, "난포란의 등급에 따른Melatonin의 첨가가 돼지 단위발생란의 체외 발달에 미치는 영향" 52-, 2015

2 Pang YW, "Treatment of porcine donor cells and reconstructed embryos with the antioxidant melatonin enhances cloning efficiency" 54 : 389-397, 2013

3 Tamura H, "The role of melatonin as an antioxidant in the follicle" 5 : 5-, 2012

4 Goud AP, "Reactive oxygen species and oocyte aging : Role of superoxide, hydrogen peroxide, and hypochlorous acid" 44 : 1295-1304, 2008

5 García JJ, "Protective effects of melatonin in reducing oxidative stress and in preserving the fluidity of biological membranes: A review" 56 : 225-237, 2014

6 Lord T, "Oxidative stress and ageing of the post-ovulatory oocyte" 146 : 217-227, 2013

7 Gupta BB, "Molecular components and mechanism of adrenergic signal transduction in mammalian pineal gland: regulation of melatonin synthesis" 43 : 115-149, 2005

8 정다운, "Melatonin의 첨가가 돼지 단위발생란의 체외 발달에 미치는 영향" 111-, 2014

9 Takada L, "Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos" 67 : 393-398, 2010

10 Shi JM, "Melatonin exists in porcine follicular fluid and improves in vitro maturation and parthenogenetic development of porcine oocytes" 47 : 318-323, 2009

11 Reiter RJ, "Melatonin and reproduction revisited" 81 : 445-456, 2009

12 정다운, "Isoproterenol의 첨가가 돼지 단위발생란의 체외 발달에 미치는 영향" 39 : 115-, 2015

13 Čikoš Š, "Expression of beta adrenergic receptors in mouse oocytes and preimplantation embryos" 71 : 145-153, 2005

14 Manjunatha BM, "Effect of taurine and melatonin in the culture medium on buffalo in vitro embryo development" 44 : 12-16, 2009

15 Kennaway DJ, "Effect of stimulation of endogenous melatonin secretion during constant light exposure on 6 sulphatoxymelatonin rhythmicity in rats" 28 : 16-25, 2000

16 Nakano M, "Effect of melatonin treatment on the developmental potential of parthenogenetic and somatic cell nuclear-transferred porcine oocytes in vitro" 20 : 199-207, 2012

17 Farahavar A, "Effect of melatonin on in vitro maturation of bovine oocytes" 9 : 2579-2583, 2010

18 Tengattini S, "Cardiovascular diseases: Protective effects of melatonin" 44 : 16-25, 2008