RBL-2H3 세포에서 Cyanidin-3-glucoside의 Th2 사이토카인 발현 억제 효과 = Suppressive Effects of Cyanidin-3-glucoside on Th2 Cytokines Production in RBL-2H3 Cells

다국어 초록 (Multilingual Abstract)

Cyanidin-3-glucoside (C3G), an anthocyanin, is one of the most widespread dietary flavonoids. We investigated
the effects of C3G in PMA/ionomycin (PI)-induced RBL-2H3 cells. C3G inhibited the production of IL-4 and IL-13 and also decreased the level of mRNA in a dose-dependent manner. Furthermore, western blot analysis implied that C3G down-reg- ulated the protein level of c-Jun, NF-ATc1 and NF-κB but not c-Fos. Taken together, we suggest that C3G may have sup- pressive effects on Th2 cytokines and will be studied further to develop as functional foods that help alleviate allergy symptoms.

목차 (Table of Contents)

• 실험방법
• 심헐결과
• 고찰 및 결론
• 감사의 말씀
• 참고문헌

참고문헌 (Reference)

1 박세봉, "유근피 추출물이 RBL-2H3 비만세포에서 β-hexosaminidase 및 cytokine 분비에 미치는 효과" 대한한방안이비인후피부과학회 24 (24): 86-95, 2011

2 Lai, Y. S, "extracts on hydrogen peroxide- and lipopolysaccharide-treated RAW264.7 macrophages" 3 : 1294-, 2012

3 Zavorotinskaya, T, "Treatment of experimental asthma by long-term gene therapy directed against IL-4 and IL-13" 7 : 155-, 2003

4 Elias, J. A, "Transgenic modeling of interleukin-13 in the lung" 123 : 339S-, 2003

5 Hogan, P. G, "Transcriptional regulation by calcium, calcineurin, and NFAT" 17 : 2205-, 2003

6 Zeng, L, "Study on anti-tumor effect of cyanidin-3-glucoside on ovarian cancer" 37 : 1651-, 2012

7 Hayden, M. S, "Signaling to NF-kappaB" 18 : 2195-, 2004

8 Li-Weber, M, "Regulation of IL4 gene expression by T cells and therapeutic perspectives" 3 : 534-, 2003

9 Passante, E, "RBL-2H3 cells are an imprecise model for mast cell mediator release" 58 : 611-, 2009

10 Qin, L, "Protective effect of cyanidin 3-O-glucoside on beta-amyloid peptide-induced cognitive impairment in rats" 534 : 285-, 2013

11 Macian, F, "Partners in transcription: NFAT and AP-1" 20 : 2476-, 2001

12 Kim, S. M, "Neuroprotective effects of black soybean anthocyanins via inactivation of ASK1-JNK/p38 pathways and mobilization of cellular sialic acids" 90 : 874-, 2012

13 Han, S. J, "Metabolism of cyanidin-3-O-beta-Dglucoside isolated from black colored rice and its antiscratching behavioral effect in mice" 74 : H253-, 2009

14 Hipskind, R. A, "MAP kinase signaling cascades and gene expression in osteoblasts" 3 : d804-, 1998

15 Gallo, E, "IL-13-producing Th1 and Th17 cells characterize adaptive responses to both self and foreign antigens" 42 : 2322-, 2012

16 Kim, L. K, "Downregulation of NF-kappaB target genes by the AP-1 and STAT complex during the innate immune response in Drosophila" 5 : e238-, 2007

17 Liang, H. E, "Divergent expression patterns of IL- 4 and IL-13 define unique functions in allergic immunity" 13 : 58-, 2012

18 최정준, "Development of an In Vitro Test System Measuring Transcriptional Downregulatory Activities on IL-13" 한국미생물·생명공학회 19 (19): 331-337, 2009

19 Schuijs, M. J, "Cytokine targets in airway inflammation" 13 : 351-, 2013

20 Speciale, A, "Cyanidin-3-O-glucoside counters the response to TNF-alpha of endothelial cells by activating Nrf2 pathway" 1-, 2013

21 Zhang, Y, "Cyanidin-3-O-beta-glucoside inhibits LPSinduced expression of inflammatory mediators through decreasing IkappaBalpha phosphorylation in THP-1 cells" 59 : 723-, 2010

22 Aketani, S, "Correlation between cytosolic calcium concentration and degranulation in RBL-2H3 cells in the presence of various concentrations of antigen-specific IgEs" 75 : 185-, 2001

23 Brusselle, G. G, "Attenuation of allergic airway inflammation in IL-4 deficient mice" 24 : 73-, 1994

24 Cui, C, "Antioxidant capacity of anthocyanins from Rhodomyrtus tomentosa (Ait.) and identification of the major anthocyanins" 139 : 1-, 2013

25 Karlsen, A, "Anthocyanins inhibit nuclear factor-kappaB activation in monocytes and reduce plasma concentrations of pro-inflammatory mediators in healthy adults" 137 : 1951-, 2007

26 Park, S. J, "Anthocyanins inhibit airway inflammation and hyperresponsiveness in a murine asthma model" 45 : 1459-, 2007

27 Johnson, M. H, "Anthocyanins and proanthocyanidins from blueberryblackberry fermented beverages inhibit markers of inflammation in macrophages and carbohydrate-utilizing enzymes in vitro" 57 : 1182-, 2013

28 Jeong, J. W, "Anthocyanins Downregulate Lipopolysaccharide- Induced Inflammatory Responses in BV2 Microglial Cells by Suppressing the NF-κB and Akt/MAPKs Signaling Pathways" 14 : 1502-, 2013

29 Jacynthe Desjardins, "Anthocyanin-Rich Black Currant Extract and Cyanidin-3-O-Glucoside Have Cytoprotective and Anti-Inflammatory Properties" 한국식품영양과학회 15 (15): 1045-1050, 2012