Arsenite Oxidation by a Facultative Chemolithotrophic Bacterium SDB1 Isolated from Mine Tailing

다국어 초록 (Multilingual Abstract)

An arsenite (As[III])-oxidizing bacterium, SDB1, was isolated from mine tailing collected from the
Sangdong mine area in Korea and showed chemolithotrophic growth on As[III] and CO2 as the respective
electron and carbon sources. SDB1 is Gram-negative, rod-shaped, and belongs to the Sinorhizobium-
Ensifer branch of α-Proteobacteria. Growth and As[III] oxidation was enhanced significantly by the presence
of yeast extract (0.005%) in minimal salt medium containing 5 mM As[III]; decreasing the doubling
time from 9.8 to 2.1 h and increasing the As[III] oxidation rate from 0.014 to 0.349 pmol As[III] oxidized
cell-1 h-1. As[III] oxidation nearly stopped at pH around 4 and should be performed at pH 7~8 to be most
effective. SDB1 was immobilized in calcium-alginate beads and the oxidation capacity was investigated.
Specific As[III] oxidation rates obtained with SDB1 (10.1~33.7 mM As[III] oxidized g-1 dry cell h-1) were
10~16-times higher than those reported previously with a heterotrophic bacterial strain (Simeonova et al.,
2005). The stability and reusability of immobilized SDB1 strongly suggested that the immobilized SDB1
cell system can make the As[III] oxidation process technically and economically feasible in practical applications.

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