Hepatitis B Virus Core Interacts with the Host Cell Nucleolar Protein, Nucleophosmin 1

다국어 초록 (Multilingual Abstract)

Hepatitis B virus (HBV) genome replication requires the packaging of viral factors (pregenomic RNA and
polymerase) as well as host factors, including heat shock proteins and protein kinase C. Previous reports
have suggested that there are several unidentified host factors that affect this encapsidation step. In this
study, we identified a new host factor, nucleophosmin (B23) that interacts with the HBV core protein 149
(Cp149). We analyzed this factor using NHS-activated sepharose resin and MALDI-TOF MS. Using the
BIAcore analysis system, we were also able to deduce that the B23.1 residues 259-294 were required for
the interaction between Cp149 and B23.1 in vitro.

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