각질형성세포가 배양된 인체 상피제거양막의 상처치유효과 = The Wound Healing Effects of Human Deepithelialized Amniotic Membrane with Skin Keratinocyte

다국어 초록 (Multilingual Abstract)

Amniotic membrane (AM) dressing can be used for the protection against superficial bacterial infection, facilitation of reepithelialization, reduction of caloric and fluid loss from burn or ulcers. In addition, it increases the speed of healing and minimizes the scarring of tissue repair of wounds. Objective of this study is to evaluate the wound healing effect of deepithelialized human AM with keratinocytes on it and the usefulness as substrates of epithelial cells culture. Deepithelialized AM was made by removal of their amniotic epithelial cells with 0.05% trypsin treatment and lyophilization and compared the wound healing effect with the intact human AM with keratinocytes. First, we evaluated the antiinflammatory effect of human AM, which was mixed with collagen solution in a powder form and freeze dried. Second, we cultured keratinocytes onto deepithelialized AM and grafted to the rat skin defect in full-thickness, and then the epidermal regeneration and dermal fibrosis was compared. As results, AM showed antiinflammatory effects evidence by much lowered inflammatory cell infiltrates in the implantation sites. Keratinocytes were cultured better and stratified on the deepithelialized AM than intact AM. Epithelial regeneration and fibrosis was also much better than intact AM. Therefore, we can conclude that AM had antiinflammatory effects, improve the wound healing and also suitable for cell culture substrates.