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      Stem-leaves of Panax as a rich and sustainable source of less-polar ginsenosides: comparison of ginsenosides from Panax ginseng, American ginseng and Panax notoginseng prepared by heating and acid treatment

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      https://www.riss.kr/link?id=A107368069

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      다국어 초록 (Multilingual Abstract)

      Background: Ginsenosides, which have strong biological activities, can be divided into polar or less-polar ginsenosides. Methods: This study evaluated the phytochemical diversity of the saponins in Panax ginseng (PG) root, American ginseng (AG) root,...

      Background: Ginsenosides, which have strong biological activities, can be divided into polar or less-polar ginsenosides.
      Methods: This study evaluated the phytochemical diversity of the saponins in Panax ginseng (PG) root, American ginseng (AG) root, and Panax notoginseng (NG) root; the stem-leaves from Panax ginseng (SPG) root, American ginseng (SAG) root, and Panax notoginseng (SNG) root as well as the saponins obtained following heating and acidification [transformed Panax ginseng (TPG), transformed American ginseng (TAG), transformed Panax notoginseng (TNG), transformed stem-leaves from Panax ginseng (TSPG), transformed stem-leaves from American ginseng (TSAG), and transformed stem-leaves from Panax notoginseng (TSNG)]. The diversity was determined through the simultaneous quantification of the 16 major ginsenosides.
      Results: The content of ginsenosides in NG was found to be higher than those in AG and PG, and the content in SPG was greater than those in SNG and SAG. After transformation, the contents of polar ginsenosides in the raw saponins decreased, and contents of less-polar compounds increased. TNG had the highest levels of ginsenosides, which is consistent with the transformation of ginseng root. The contents of saponins in the stem-leaves were higher than those in the roots. The transformation rate of SNG was higher than those of the other samples, and the loss ratios of total ginsenosides from NG (6%) and SNG (4%) were the lowest among the tested materials. In addition to the conversion temperature, time, and pH, the crude protein content also affects the conversion to rare saponins. The proteins in Panax notoginseng allowed the highest conversion rate.
      Conclusion: Thus, the industrial preparation of less-polar ginsenosides from SNG is more efficient and cheaper.

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      목차 (Table of Contents)

      • ABSTRACT
      • 1. Introduction
      • 2. Materials and methods
      • 3. Results and discussion
      • 4. Conclusions
      • ABSTRACT
      • 1. Introduction
      • 2. Materials and methods
      • 3. Results and discussion
      • 4. Conclusions
      • References
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      참고문헌 (Reference)

      1 백인호, "The world ginseng market and the ginseng (Korea)" 고려인삼학회 37 (37): 1-7, 2013

      2 Qu CL, "Study on ginsenosides in different parts and ages of Panax quinquefolius L" 115 : 340-346, 2009

      3 Qu CL, "Studies on fragmentation pathways of amino acids and their interactions with ginsenoside Rb3 by mass spectrometry" 29 : 1721-1726, 2008

      4 Yang Xiu, "Simultaneous determination and difference evaluation of 14 ginsenosides in Panax ginseng roots cultivated in different areas and ages by high-performance liquid chromatography coupled with triple quadrupole mass spectrometer in the multiple reactionemonitoring mode combined with multivariate statistical analysis" 고려인삼학회 43 (43): 508-516, 2019

      5 Wang CZ, "Saponins composition in American ginseng leaf and berry assayed by high-performance liquid chromatography" 54 : 2261-2266, 2006

      6 Xin-fang Xu, "Remarkable impact of steam temperature on ginsenosides transformation from fresh ginseng to red ginseng" 고려인삼학회 42 (42): 277-287, 2018

      7 Zhi Liu, "Remarkable impact of amino acids on ginsenoside transformation from fresh ginseng to red ginseng" 고려인삼학회 44 (44): 424-434, 2020

      8 Liu Z, "Remarkable impact of acidic ginsenosides and organic acids on ginsenoside transformation from fresh ginseng to red ginseng" 64 : 5389-5399, 2016

      9 Sun S, "Red notoginseng : higher ginsenoside content and stronger anticancer potential than Asian and American ginseng" 125 : 1299-1305, 2011

      10 Quan K, "Rapid preparation of rare ginsenosides by acid transformation and their structureactivity relationships against cancer cells" 5 : 8598-, 2015

      1 백인호, "The world ginseng market and the ginseng (Korea)" 고려인삼학회 37 (37): 1-7, 2013

      2 Qu CL, "Study on ginsenosides in different parts and ages of Panax quinquefolius L" 115 : 340-346, 2009

      3 Qu CL, "Studies on fragmentation pathways of amino acids and their interactions with ginsenoside Rb3 by mass spectrometry" 29 : 1721-1726, 2008

      4 Yang Xiu, "Simultaneous determination and difference evaluation of 14 ginsenosides in Panax ginseng roots cultivated in different areas and ages by high-performance liquid chromatography coupled with triple quadrupole mass spectrometer in the multiple reactionemonitoring mode combined with multivariate statistical analysis" 고려인삼학회 43 (43): 508-516, 2019

      5 Wang CZ, "Saponins composition in American ginseng leaf and berry assayed by high-performance liquid chromatography" 54 : 2261-2266, 2006

      6 Xin-fang Xu, "Remarkable impact of steam temperature on ginsenosides transformation from fresh ginseng to red ginseng" 고려인삼학회 42 (42): 277-287, 2018

      7 Zhi Liu, "Remarkable impact of amino acids on ginsenoside transformation from fresh ginseng to red ginseng" 고려인삼학회 44 (44): 424-434, 2020

      8 Liu Z, "Remarkable impact of acidic ginsenosides and organic acids on ginsenoside transformation from fresh ginseng to red ginseng" 64 : 5389-5399, 2016

      9 Sun S, "Red notoginseng : higher ginsenoside content and stronger anticancer potential than Asian and American ginseng" 125 : 1299-1305, 2011

      10 Quan K, "Rapid preparation of rare ginsenosides by acid transformation and their structureactivity relationships against cancer cells" 5 : 8598-, 2015

      11 Quan K, "Rapid preparation of rare ginsenosides by acid transformation and their structureactivity relationships against cancer cells" 5 : 85-98, 2015

      12 Wang JR, "Quantitative comparison and metabolite profiling of saponins in different parts of the root of Panax notoginseng" 62 : 9024-9034, 2014

      13 Fang Liu, "Preparative separation of minor saponins from Panax notoginseng leaves using biotransformation, macroporous resins, and preparative high-performance liquid chromatography" 고려인삼학회 43 (43): 105-115, 2019

      14 Xia J, "Preparation of rare ginsenoside by trans -formation of ginsenoside Re catalyzed transformation of ginsenoside Re catalyzed with aspartic acid" 19 : 3389-3394, 2016

      15 Jo SK, "Preparation of ginsenosides Rg3, Rk1, and Rg5-selectively enriched ginsengs by a simple steaming process" 240 : 251-256, 2015

      16 Li FL, "Pharmacology effect of ginsenosides from stems and leaves of Panax ginseng were same like ginsenosides from roots" 41 : 54-57, 2013

      17 Lei Cui, "Microbial conversion of major ginsenosides in ginseng total saponins by Platycodon grandiflorum endophytes" 고려인삼학회 40 (40): 366-374, 2016

      18 Ma LY, "Metabolism of 20(S)-Ginsenoside Rg2 by rat liver microsomes : bioactivation to SIRT1-activating metabolites" 21 : 757-772, 2016

      19 Wang S, "Maldi imaging for the localization of saponins in root tissues and rapid differentiation of three panax herbs" 37 : 1956-1966, 2016

      20 Bai H, "Localization of ginsenosides in panax ginseng with different age by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry imaging" 1026 : 263-271, 2015

      21 Kwon SW, "Liquid chromatographic determination of less polar ginsenosides in processed ginseng" 921 : 335-339, 2001

      22 Hoang Tung Vo, "Kinetic study for the optimization of ginsenoside Rg3 production by heat treatment of ginsenoside Rb1" 고려인삼학회 39 (39): 304-313, 2015

      23 Gwi Yeong Jang, "Influence of organic acids and heat treatment on ginsenoside conversion" 고려인삼학회 42 (42): 532-539, 2018

      24 Peng Xue, "Improved antimicrobial effect of ginseng extract by heat transformation" 고려인삼학회 41 (41): 180-187, 2017

      25 Yang W, "Identification and differentiation of panax ginseng, panax quinquefolium, and panax notoginseng by monitoring multiple diagnostic chemical markers" 6 : 568-575, 2016

      26 Du J, "Identification and characterization of a ginsenoside-transforming b-glucosidase from Pseudonocardia sp. Gsoil 1536 and its application for enhanced production of minor ginsenoside Rg2(S)" 9 : e96914-, 2014

      27 Zhang CX, "HPLC determination of the amount of ginsenosides in different part of Panax ginseng C.A.Mey. and P. quinquefolius L. and P. notoginseng (burk) F.H.Che" 25 : 1190-1194, 2005

      28 Popovich DG, "Generation of ginsenosides Rg3 and Rh2 from North American ginseng" 65 : 337-344, 2004

      29 Huq MA, "Enzymatic transformation of ginseng leaf saponin by recombinant b-glucosidase (bgp1) and its efficacy in an adipocyte cell line" 63 : 532-538, 2015

      30 Jitendra Upadhyaya, "Enzymatic formation of compound-K from ginsenoside Rb1 by enzyme preparation from cultured mycelia of Armillaria mellea" 고려인삼학회 40 (40): 105-112, 2016

      31 Le THV, "Effects of steaming on saponin compositions and anti-proliferative activity of Vietnamese ginseng" 35 (35): 274-278, 2015

      32 조형택, "Effects of Panax ginseng extracts prepared at different steaming times on thermogenesis in rats" 고려인삼학회 41 (41): 347-352, 2017

      33 류제빈, "Effect of hydrothermal processing on ginseng extract" 고려인삼학회 41 (41): 572-577, 2017

      34 Li F, "Effect of different drying methods on ginsenosides in flower of panax ginseng and panax quinquefolius" 46 : 2937-2942, 2015

      35 Kamizake NKK, "Determination of total proteins in cow milk powder samples: a comparative study between the Kjeldahl method and spectrophotometric methods" 16 : 507-516, 2003

      36 Zhang L, "Determination of the transformation of ginsenosides in Ginseng Radix et Rhizoma during decoction with water using ultrafast liquid chromatography coupled with tandem mass spectrometry" 41 : 1039-1049, 2018

      37 Xi MF, "Determination of content of total saponins in Panpax Notoginseng roots and leaves by aqueous two phase system" 31 : 24-27, 2010

      38 Liu Z, "Degradation of protopanaxadioltype ginsenosides with aspartic acid and antioxidant activity of Maillard reaction products" 39 : 20-26, 2017

      39 Lee JW, "Comprehensive profiling and quantification of ginsenosides in the root, stem, leaf, and berry of Panax ginseng by UPLC-QTOF/MS" 22 : 2147-2159, 2017

      40 Ill-Min Chung, "Comparative phenolic compound profiles and antioxidative activity of the fruit, leaves, and roots of Korean ginseng (Panax ginseng Meyer) according to cultivation years" 고려인삼학회 40 (40): 68-75, 2016

      41 Mi Ra Lee, "Comparative Study of White and Steamed Black Panax ginseng, P. quinquefolium, and P. notoginseng on Cholinesterase Inhibitory and Antioxidative Activity" 고려인삼학회 36 (36): 93-101, 2012

      42 Ru-Feng Wang, "Chemical transformation and target preparation of saponins in stems and leaves of Panax notoginseng" 고려인삼학회 42 (42): 270-276, 2018

      43 Wan JB, "Chemical investigation of saponins in different parts of Panax notoginseng by pressurized liquid extraction and liquid chromatography-electrospray ionization-tandem mass spectrometry" 17 : 5836-5853, 2012

      44 Cho Rong Hwang, "Changes in ginsenoside compositions and antioxidant activities of hydroponic-cultured ginseng roots and leaves with heating temperature" 고려인삼학회 38 (38): 180-186, 2014

      45 김신정, "Changes in ginsenoside composition of ginseng berry extracts after a microwave and vinegar process" 고려인삼학회 37 (37): 269-272, 2013

      46 Jung-Hyun Yi, "Change of Ginsenoside Composition in Red Ginseng Processed with Citric Acid" 한국식품과학회 19 (19): 647-653, 2010

      47 Luo SL, "Biotransformation of saponins by endophytes isolated from Panax notoginseng" 10 : 2021-2031, 2013

      48 Wan JY, "Biotransformation and metabolic profile of American ginseng saponins with human intestinal microflora by liquid chromatography quadrupole time-of-flight mass spectrometry" 1286 : 83-92, 2013

      49 Kim JK, "Bioconversion of major ginsenosides Rg1, to minor ginsenoside F1, using novel recombinant ginsenoside hydrolyzing glycosidase cloned from Sanguibacter keddieii, and enzyme characterization" 161 : 294-301, 2012

      50 Xue P, "Antifungal activity and mechanism of heattransformed ginsenosides from notoginseng against Epidermophyton floccosum, Trichophyton rubrum, and Trichophyton mentagrophyte" 7 : 10939-10946, 2017

      51 Duan Z, "Anticancer effects of ginsenoside Rk3 on non-small cell lung cancer cells : in vitro and in vivo" 2017

      52 Shin-Jung Kim, "Anti-breast cancer activity of Fine Black ginseng (Panax ginseng Meyer) and ginsenoside Rg5" 고려인삼학회 39 (39): 125-134, 2015

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