Production of β-carotene by Recombinant Escherichia coli with Engineered Whole Mevalonate Pathway in Batch and Fed-batch Cultures

다국어 초록 (Multilingual Abstract)

Recombinant Escherichia coli engineered to contain the whole mevalonate pathway and foreign genes for β-carotene biosynthesis,
was utilized for production of β-carotene in bioreactor cultures. Optimum culture conditions were established in
batch and pH-stat fed-batch cultures to determine the optimal feeding strategy thereby improving production yield. The specific
growth rate and volumetric productivity in batch cultures at 37oC were 1.7-fold and 2-fold higher, respectively, than
those at 28oC. Glycerol was superior to glucose as a carbon source. Maximum β-carotene production (titer of 663 mg/L and
overall volumetric productivity of 24.6 mg/L × h) resulted from the simultaneous addition of 500 g/L glycerol and 50 g/L yeast
extract in pH-stat fed-batch culture.

다국어 초록 (Multilingual Abstract)

Recombinant Escherichia coli engineered to contain the whole mevalonate pathway and foreign genes for β-carotene biosynthesis,
was utilized for production of β-carotene in bioreactor cultures. Optimum culture conditions were established in
batch and pH-stat fed-batch cultures to determine the optimal feeding strategy thereby improving production yield. The specific
growth rate and volumetric productivity in batch cultures at 37oC were 1.7-fold and 2-fold higher, respectively, than
those at 28oC. Glycerol was superior to glucose as a carbon source. Maximum β-carotene production (titer of 663 mg/L and
overall volumetric productivity of 24.6 mg/L × h) resulted from the simultaneous addition of 500 g/L glycerol and 50 g/L yeast
extract in pH-stat fed-batch culture.

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