분리된 흰쥐 간 핵속의 핵단백질의 인산화반응에 미치는 싸이클릭 뉴클레오티드와 인슐린의 영향 = Effects of Cyclic nucleotides and insulin on In Vitro Phosphorylation of Nuclear Proteins in isolated Rat Liver Nuclei

다국어 초록 (Multilingual Abstract)

The effects of cyclic nucleotides and insulin on the phosphorylation patterns of nuclear proteins were investigated by SDS polyacrylamide gel electrophoresis analysis following incubation of livar nuclei isolated from normal fed rats with (γ-^(32)P] ATP. Both c-AMP and c-GMP increased markedly the phosphorylation levels of nucleoplasmic proteins with molecular weights above 53,000 daltons while little change was observed with insulin. The effect of c-GMP was much greater than that of c-AMP. On the other hand, cyclic nucleotides and insulin somewhat inhibited rather phosphorylation of nuclear proteins tightly bound to chromatin such as histones and phenol soluble non-histone proteins.