忍冬藤이 DNCB로 유발된 알레르기성 접촉피부염에 미치는 영향 = Effects of Lonicerae Caulis (LC) on Allergic Contact Dermatitis (ACD) induced by DNCB in mice

다국어 초록 (Multilingual Abstract)

Objectives : Allergic contact dermatitis(ACD) is a type IV delayed hypersensitivity reaction that results from cumulative exposures and subsequent sensitization to an environmental chemical. Lonicerae Caulis(LC) can clear away heat and relieve toxin, disperse wind and heat, dredge the channel. The present study was designed to investigate the effects of LC on allergic contact dermatitis(ACD) induced by 2,4-dinitrochlorobenzene(DNCB) in mice.
Methods : In this experiment, the effects of LC on changes in body weights, ear and dorsum skin thicknesses, ear weights, clinical aspects on the dorsum skin, histopathological changes, spleen/body ratio, cytokines were investigated. In addition, the effects on proliferations of splenocytes were also investigated in vitro and vivo study.
Results : LC spread(SPR) group and LC spread and administered(SPR+ADM) group showed diminished ear thicknesses. In SPR+ADM group, ear weights were lowered significantly compared to contact dermatitis control(CTL) group. LC treatment diminished erythema, desquamation and keratosis which were induced by repeated painting of DNCB. In histopathological observation, spongiosis and edema were diminished in SPR and SPR+ADM group. In cytokines, SPR+ADM group were increased in IL-10, and SPR and SPR+ADM group were decreased in TNF-α compared with control group.
Conclusions : These data suggest that LC can decrease symptoms of ACD, then LC is useful to treat patient with ACD.

참고문헌 (Reference)

1 이지현, "아토피피부염학회 보고서: 한국 아토피피부염의 치료지침" 대한피부과학회 44 (44): 907-913, 2006

2 정인조, "개에서 실험적으로 유발한 비장종대의 방사선학적 평가" 한국임상수의학회 23 (23): 263-266, 2006

3 이영철, "忍冬藤의 免疫調節作用에 대한 實驗的 硏究(Ⅱ)" 대한본초학회 20 (20): 141-149, 2005

4 Lobell A, "Vaccination with DNA encoding an immunodominant myelin basic protein peptide targeted to Fc of immunoglobulin G suppresses experimental autoimmune encephalomyelitis" 187 (187): 1543-1548, 1998

5 Oh G, "Uijonggeumgam" Liáonínggwahakgisulchulpansa 649-, 1997

6 Jeon JH, "The literature study on Contact dermatitis" 10 (10): 263-283, 1997

7 Jeon JH, "The literature study on Contact dermatitis" 11 (11): 197-218, 1998

8 Kim MH, "The antioxidative effects of the water-soluble extracts of plants used as tae materials" 33 : 12-18, 2001

9 Korean Dermatological Association Textbook Compilation Committee, "Text of Dermatology" Yeomoongak 63-84, 2008

10 So WB, "Sossiceypyengwenhwulon" Daesung P CO 258-, 1992

11 Burk RL, "Small animal radiology and ultrasonography: a diagnostic atlas and text" WB Saunders Co 410-414, 1996

12 Zhao J, "Seongjechongnok" Inminwisaengchulpansa 1577-1579, 1995

13 Mizel SB, "Regulation of Lymphocyte-Activating Factor(LAF)Production and Secretion in P388D1 Cells : Identification of High Molecular Weight Precursors of LAF" 122 (122): 2173-2179, 1979

14 Okura Y, "Recombinant murine interleukin-12 facilitates induction of cardiac myosin-specific type 1 helper T cells in rats" 82 (82): 1035-1042, 1998

15 Wysocki LJ, "Planning for lymphocytes:a method for cell selection" 75 (75): 2844-2848, 1978

16 Jin SG, "Oegwajeongjong" Inminwisaengchulpansa 259-, 1983

17 Jeon KW, "Junguioegwahak" Junguigojeokchulpansa 340-342, 1994

18 Rivera DL, "Interleukin-10attenuates experimental fetal growth restriction and demise" 12 (12): 189-197, 1998

19 Kim JA, "Inhibitory effect of luteolin on TNF-α-induced IL-8 production in human colon epithelial cells" 5 (5): 209-217, 2005

20 하태광, "Immunomodulatory effects of chlorogenic acid and ethyl acetate fraction from Lonicera japonica on cytokine gene expression profiles in spleen and thymus" 대한본초학회 26 (26): 1-10, 2011

21 Dayer JM, "Human recombinant interleukin 1 stimulates collagenase and prostaglandin E2 production by human synovial cells" 77 (77): 645-648, 1986

22 Maeda A, "Experimental extracorporeal photopheresis inhibits the sensitization and effector phases of contact hypersensitivity via two mechanisms : generation of IL-10 and induction of regulatory T cells" 181 (181): 5956-5962, 2008

23 Corsini E, "Epidermal cytokines in experimental contact dermatitis" 142 (142): 203-211, 2000

24 서영배, "Effects of Ethyl Acetate Fraction of ELC on Highly Purified Mice T cells" 대한본초학회 18 (18): 14-129, 2003

25 Yuan XY, "Effects and mechanisms of aloperine on 2, 4-dinitrofluorobenzene-induced allergic contact dermatitis in BALB/c mice" 629 (629): 147-152, 2010

26 Kim JK, "Effect of Scrophularia buergeriana Extract on the Degranulation of Mast Cells and Ear Swelling Induced by Dinitrofluorobenzene in Mice" 35 (35): 183-191, 2012

27 Heo J, "Donguibogam" Donguibogamchulpansa 196-, 2005

28 Jin ST, "Dongcheonchongnok" Inminwisaengchulpansa 148-, 1999

29 Lee SJ, "Development of Plant Anti-inflammatory Agents : Comparison of Anti-inflammatory and Analgesic Activities of Extracts from Lonicera japonica" 25 (25): 363-367, 1994

30 Ahn SG, "Common Skin Diesease" Doctor's book 173-190, 2009

31 Stasiuk LM, "Collagen-Induced Arthritis in DBA/1 Mice : Cytokine Gene Activation Following Immunization with Type II Collagen" 173 (173): 269-275, 1996

32 Textbook publishing Committee of Korean Medicine, "Bonchohak" Younglimsa 267-268, 2004

33 Chio KU, "Asthma and air polltion in Korea" 17 : 64-75, 1995

34 Tae J, "Anti-inflammatory effect of Lonicera japonica in proteinase-activated receptor 2-mediated paw edema" 330 (330): 165-171, 2003

35 Lee SJ, "Anti-inflammatory Actibity of the Major Constituents of Lonicera japonica" 18 (18): 133-135, 1995

36 Kim SS, "Anti-cancer and whitening effects of Lonicerae Caulis (LC) in melanoma cells" Graduate school of Dongshin University 2010

37 Ryu KH, "Anti-asthmatic Activities of the Extract of Lonicera japonica" 30 (30): 377-383, 1999

38 Jung GM, "Allergy and Korean medicine" Jeillo 46-49, 1993

39 Saint MP, "Allergic contact dermatitis" 14 (14): 284-295, 2004

40 Stepaniak JA, "A comparative study of experimental autoimmune encephalomyelitis in Lewis and DA rats" 155 (155): 2762-2769, 1995