Amelioratory Effects of Acorn Mook Ethanol Extract on ROS Production and Lipid Accumulation in Differentiated 3T3-L1 Cells

다국어 초록 (Multilingual Abstract)

Obesity is a cause of various metabolic diseases, so prevention and treatment are important. Recently, there has been an increasing interest in natural foods that promote fat breakdown or inhibit fat accumulation. The objective of this study was to investigate the amelioratory effect of acorn and acorn mook ethanol extract on reactive oxygen species (ROS) production and lipid accumulation in differentiated 3T3-L1 cells. First, antioxidant activity and the expression of C/EBP-β, C/EBP-α, PPAR-γ, and aP2 level by western blotting in 3T3-L1 cells of acorn ethanol extract were examined. Total polyphenol and total flavonoid contents of acorn ethanol extract were 476.44±14.70 mg TAE/g and 102.91±13.14 mg QE/g, respectively. AEtOH suppressed C/EBP-β, C/EBP-α, PPAR-γ expression, an adipogenesis related transcription factor, and inhibited aP2 expression in 3T3L-1 cells. Based on the first results, we made acorn mook and treated 3T3-L1 cells with acorn mook ethanol extract to investigate its inhibitory effect on ROS production and lipid accumulation. Acorn mook ethanol extract significantly inhibited ROS production and lipid accumulation in 3T3-L1 cells. These results suggest that acorn mook might be an effective potential agent to ameliorate obesity.