High-level Production of Ethanol during Fed-batch Ethanol Fermentation with a Controlled Aeration Rate and Non-sterile Glucose Powder Feeding of Saccharomyces cerevisiae

다국어 초록 (Multilingual Abstract)

In this study, we utilized a unique strategy for fed-batch fermentation using ethanol-tolerant Saccharomyces cerevisae toachieve a high-level of ethanol production that could be practically applied on an industrial scale. During this study, theaeration rate was controlled at 0.0, 0.13, 0.33, and 0.8 vvm to determine the optimal aeration conditions for the productionof ethanol. Additionally, non-sterile glucose powder was fed during fed-batch ethanol fermentation and corn-steepliquor (CSL) in the medium was used as an organic N-source. When aeration was conducted, the ethanol productionand productivity were superior to that when aeration was not conducted. Specifically, the maximum ethanol productionreached approximately 160 g/L, when the fermentor was aerated at 0.13 vvm. These findings indicate that the use of amuch less expensive C-source may enable the fermentation process to be directed towards the improvement of overallethanol production and productivity in fermentors that are aerated at 0.13 vvm. Furthermore, if a repeated fed-batchprocess in which the withdrawal and fill is conducted prior to 36 h can be employed, aeration at a rate of 0.33 and/or 0.8vvm may improve the overall ethanol productivity

참고문헌 (Reference)

1 Sánchez, Ó. J., "Trends in biotechnological production of fuel ethanol from different feedstocks" 99 : 5270-5295, 2008

2 Herman, P. K., "Stationary phase in yeast" 5 : 602-607, 2002

3 Gray, J. V., "Sleeping beauty: quiescence in Saccharomyces cerevisiae" 68 : 187-206, 2004

4 Cardoso, H., "Sequential inactivation of ammonium and glucose transport in Saccharomyces cerevisiae during fermentation" 73 : 155-159, 1992

5 Demirbas, A., "Progress and recent trends in biofuels" 33 : 1-18, 2007

6 Thomas, K. C., "Production of fuel alcohol from wheat by VHG technology" 43 : 211-226, 1993

7 Thomas, K. C., "Production of fuel alcohol from wheat by VHG technology" 43 : 211-226, 1993

8 Thomas, K. C., "Production of 21% (v/v) ethanol by fermentation of very high gravity (VHG) wheat mashes" 10 : 61-68, 1992

9 Krishnan, M. S., "Process engineering of high-ethanol-tolerance yeast for the manufacture of ethanol" 51 : 479-493, 1995

10 Cot, M., "Physiological behaviour of Saccharomyces cerevisiae in aerated fed-batch fermentation for high level production of bioethanol" 7 : 22-32, 2007

11 Bai, F. W., "Parameter oscillations in very high gravity medium continuous ethanol fermentation and their attenuation on multi-stage packed column bioreactor system" 88 : 558-566, 2004

12 Feng-Wu Bai, "Observed Quasi-steady Kinetics of Yeast Cell Growth and Ethanol Formation under Very High Gravity Fermentation Condition" 한국생물공학회 10 (10): 115-121, 2005

13 Jung, K. -H., "Methyl α-D-glucopyranoside enhances the enzymatic activity of recombinant β-galactosidase inclusion bodies in the araBAD promoter system of Escherichia coli" 35 : 695-701, 2008

14 Alfenore, S., "Improving ethanol production and viability of Saccharomyces cerevisiae by a vitamin feeding strategy during fed-batch process" 60 : 67-72, 2002

15 Bafrncona, P., "Improvement of very high gravity ethanol fermentation by media supplementation using Saccharomyces cerevisiae" 21 : 337-341, 1999

16 Casey, G. P., "High-gravity brewing: nutrient enhanced production of high concentrations of ethanol by brewing yeast" 5 : 429-434, 1983

17 Wang, S., "Grain pearling and very high gravity (VHG) fermentation technologies for fuel alcohol production from rye and triticale" 34 : 421-428, 1999

18 Cardona, C. A., "Fuel ethanol production: process design trends and integration opportunities" 98 : 2415-2457, 2007

19 Jones, A. M., "Fuel ethanol production: appraisal of nitrogeneous yeast foods for very high gravity wheat mash fermentation" 29 : 483-488, 1994

20 Thomas, K. C., "Fuel alcohol production: effects of free amino nitrogen on fermentation of very-high-gravity wheat mash" 56 : 2046-2050, 1990

21 Hayashida, S., "Formation of high concentrations of alcohol by various yeasts" 87 : 42-44, 1981

22 Hamelinck, C. N., "Ethanol from lignocellulosic biomass: technoeconomic performance in short-, middle-, and long-term" 28 : 384-410, 2005

23 최기욱, "Ethanol Production by Zymomonas mobilis CHZ2501 from Industrial Starch Feedstocks" 한국생물공학회 13 (13): 765-771, 2008

24 Jung, Kyung-Hwan, "Enhanced Enzyme Activities of Inclusion Bodies of Recombinantβ-Galactosidase via the Addition of Inducer Analog after L-ArabinoseInduction in the araBAD Promoter System of Escherichia coli" 한국미생물·생명공학회 18 (18): 434-442, 2008

25 Leão, C., "Effects of ethanol and other alkanols on the glucose transport system of Saccharomyces cerevisiae" 24 : 2601-2604, 1982

26 Leão, C., "Effects of ethanol and other alkanols on passive proton influx in the yeast Saccharomyces cerevisiae" 774 : 43-48, 1984

27 안대희, "Development of a Serial Bioreactor Systemfor Direct Ethanol Production from StarchUsing Aspergillus niger and Saccharomyces cerevisiae" 한국생물공학회 12 (12): 566-573, 2007

28 Bai, F. W., "Continuous ethanol production and evaluation of yeast cell lysis and viability loss under very high gravity medium conditions" 110 : 287-293, 2004

29 Chaplin, M. F., "Carbohydrate analysis: A practical approach" IRL Press 3-, 1986

30 Shuler, M. L., "Bioprocess engineering, Basic concepts. 2nd ed" Prentice-Hall Inc. 292-297, 2002

31 Atkinson, B., "Biochemical engineering and biotechnology handbook" The nature press 772-773, 1983

32 Alfenor, S., "Aeration strategy: a need for very high ethanol performance in Saccharomyces cerevisiae fed-batch process" 63 : 537-542, 2004

33 Mota, M., "A simple device for fed-batch control in alcoholic fermentation" 24 : 775-777, 1987