Resveratrol의 CD4+ T 세포 활성과 분화 억제 효과 = Resveratrol Suppresses CD4+ T Cell Activation and Differentiation in vitro

다국어 초록 (Multilingual Abstract)

Resveratrol is a naturally occurring stilbene which is safe and well-described compound with a potent anti-inflammatory activity. Recent studies suggested that resveratrol suppressed various inflammation mediated diseases such as asthma, chronic colitis, rheumatoid arthritis, and type 1 diabetes. These studies indicated that resveratrol might directly modulate CD4 + helper T cells (Th cells)-mediated immune responses. However, it is not fully elucidated whether resveratrol directly regulates CD4 + Th cell activation and differentiation. In the present study, CD4 + Th cells were purified from C57BL/6 and treated with various concentrations of resveratrol. We found that resveratrol directly suppressed CD4 + Th cells activation, leading to a defect in T cell proliferation. When CD4 + Th cells were treated with resveratrol, cytokine production was also significantly reduced in a dose dependent manner. In accordance with these results, resveratrol even inhibited CD4 + Th cells differentiation into Th1, Th2 or Th17, which produces IFN-γ, IL-4 or IL-17 respectively. We also found that resveratrol could induce apoptosis of CD4 + T cells at a high concentration. Our data demonstrated that resveratrol inhibited directly CD4 + Th cells activation and differentiation. It suggests that resveratrol could be an efficient therapeutic strategy for autoimmune diseases in which CD4 + Th cells play a critical role.

국문 초록 (Abstract)

Resveratrol은 천연 stilbene으로 안전성 있는 항염증 활성을 가진 화합물로 알려져 있다. 최근의 연구들에서 resveratrol이 천식, 만성 대장염, 류마티스성 관절염과 같이 염증에 의해발생하는 다양한 질병을 억제한다고 보고되었다. 이러한 연구들은 resveratrol이 CD4+ helper T cells (Th cells)에 의한 면역반응을 조절할 것이라고 제시하였다. 그러나 resveratrol이 직접적으로 Th cells의 활성화와 분화를 조절하는지 완전히 밝혀지지 않았다. C57BL/6에서 Th cells을 분리하여 다양한 농도의resveratrol을 세포에 처리하였다. 본 연구에서는 resveratrol이 직접적으로 Th cells의 활성화와 증식을 억제하는 것을 확인하였다. Th cells에 resveratrol을 처리하였을 때 IFN-γ, IL-4,IL-17 사이토카인 생성이 농도에 따라 유의하게 감소하였고 또한 Th cells이 이러한 사이토카인들을 분비하는 Th1과 Th2과Th17으로 분화되는 것이 억제되었다. 그리고 고농도의 resveratrol이 Th cells의 세포사멸을 유도하는 것으로 확인되었다. 본 연구에서는 resveratrol이 Th cells의 활성화와 분화를 직접적으로억제하는 것을 확인하였으며, 이는 resveratrol이 CD4+ Thcells에 의해 발생되는 자가면역질환의 효과적인 치료법이 될수 있을 것이라고 제시한다.

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