Determination of 4-Methylimidazole and 2-Acetyl-4(5)-tetrahydroxybutylimidazole in Caramel Color and Processed Foods by LC-MS/MS

다국어 초록 (Multilingual Abstract)

In this study, the quick HPLC-MS/MS method for the simultaneous separation of 2-acetyl-4(5)-tetrahydroxybuthylimidazole (THI) and 4-(5)-methylimidazole (4-MI) in alkaline medium was used for caramel color and processedfoods in Korea. After a simple sample pretreatment, 51 4-MI-labeled samples were positive for 4-MI and 2 also contained THI. The concentration of 4-MI was 260.5∼24,499.3 μg/kg in caramel color, less than LOD∼1,712.5 μg/kg in sauce, 1,242.3, 5,972.2 μg/kg in balsamic vinegar, 2,118.3∼5,802.4 μg/kg in complex seasoning, 82.7∼5,110.6 μg/kg in curry, and 29.9∼464.4 μg/kg in soft drinks. The recovery rate of 4-MI was 97.1∼111.0% in sauce and 81.9∼110.0% in powder and that of THI was 83.6∼106.4% in sauce and 61.2∼99.4% in powder. Our results concluded a safe amount of 4-MI and THI compared to the limit of Korea additive code but the processed foods do not have a limit of caramel color and 4-MI in Korea. Therefore, research and monitoring of 4-MI and THI is needed for processed foods in Korea.

참고문헌 (Reference)

1 Mackenzie KM, "Toxicity studies of caramel colour III and 2-acetyl-4(5)-tetrahydroxybuylimidazole in F344 rats" 30 : 417-425, 1992

2 Chan PC, "Toxicity and carcinogenicity studies of 4-methylimidazole in F344/N rats and B6C3F1 mice" 82 : 45-53, 2008

3 Lojkova L, "Supercritical fluid extraction(SFE)of 4(5)-methylimidazole(4-Mel)and 2-acetyl-4(5)-(1, 2, 3, 4)-tetrahydroxybutyl-imidazole(THI)from ground-coffee with high-performance liquid chromatographic-electrospray mass spectrometric quantification(HPLC/ESI-MS)" 23 : 963-973, 2006

4 Klejdus B, "Solid-phase extraction of 4(5)-methylimidazole (4MeI) and 2-acetyl-4(5)-(1,2,3,4-tetrahydroxybutyl)-imidazole (THI) from foods and beverages with subsequent liquid chromatographic- electrospray mass spectrometric quantification" 29 : 378-384, 2006

5 European Food Safety Authority, "Scientific opinion on the re-evaluation of caramel colours(E 150 a, b, c, d)as food additives" 9 : 103-, 2011

6 OEHHA, "No significant risk level (NSRL) for the proposition 65 carcinogen 4-methylimidazole. Reproductive and Cancer Hazard Assessment Branch Office of Environmental Health Hazard Assessment (OEHHA) California Environmental Protection Agency"

7 MFDS, "Korea Food Additives Code. Ministry of Food and Drug Safety"

8 Chan P, "Induction of thyroid lesions in 14-week toxicity studies of 2 and 4-methylimidazole in Fisher 344/N rats and B6C3F1 mice" 80 : 169-180, 2006

9 IARC, "IARC Working Group on the Evaluation of Carcinogenic Risks to Humans. International Agency for Research on Cancer"

10 Casal S, "Gas chromatographic-mass spectrometric quantification of 4-(5)-methylimidazole in roasted coffee after ion-pair extraction" 976 : 285-291, 2002

11 Yamaguchi H, "Determination of 4(5)- methylimidazole in soy sauce and other foods by LC-MS/MS after solid-phase extraction" 59 : 9770-9775, 2011

12 Schlee C, "Determination of 2-methylimidazole, 4-methylimidazole and 2-acetyl-4-(1, 2, 3, 4-tetrahydroxy butyl)imidazole in caramel colours and cola using LC/MS/ MS" 927 : 223-226, 2013

13 Cunh SC, "Assessment of 4-(5)-methylimidazole in soft drinks and dark beer" 24 : 609-614, 2011