금창초 캘러스 추출물 및 금창초 생물전환 캘러스 추출물의 항산화, 미백 효과

다국어 초록 (Multilingual Abstract)

As the concept of natural and organic cosmetics is included in the Korean Cosmetics Act, it is necessary to find natural derived cosmetic ingredient which have efficacy and stability. In this study, we investigated the antioxidant and anti-melanogenic activities of Ajuga decumbens callus extract and biorenovated Ajuga decumbens callus extract. The antioxidant activities were measured through DPPH, ABTS scavenging assay and FRAP assay. We investigated the reduction activity of the intracellular ROS using DCF-DA fluorescence and we further researched the cytoprotective effect. To investigate the whitening effect we conducted tyrosinase inhibition assay, L-DOPA oxidation inhibition assay and melanin synthesis assay on B16f10 melanoma cell. Ajuga decumbens callus extract and biorenovated Ajuga decumbens callus extract showed strong antioxidant activities on DPPH, ABTS radical scavenging assay. Furthermore, Ajuga decumbens callus extract and biorenovated Ajuga decumbens callus extract decreased the intracellular ROS on skin normal cells. Ajuga decumbens callus extract and biorenovated Ajuga decumbens callus extract also increased the cell viabilities reduced by 2,2-azobis dihydrochloride(AAPH). Ajuga decumbens callus extract and biorenovated Ajuga decumbens callus extract showed inhibition activites of tyrosinase, L-DOA oxidation. Ajuga decumbens callus extract and biorenovated Ajuga decumbens callus extract also inhibited melanin synthesis on B16F10 melanoma cell. Based on these result, we could propose Ajuga decumbens callus extract and biorenovated Ajuga decumbens callus extract are the natural cosmetic ingredients which have antioxidant, whitening properties.

목차 (Table of Contents)

• ABSTRACT
• I. 서론
• II 재료 및 방법
• III 결과 및 고찰
• IV 결론
• 참고문헌

참고문헌 (Reference)

1 박서연, "천연화장품에 대한 인식과 사용실태 및 만족도에 관한 연구" 한국화장품미용학회 10 (10): 157-171, 2020

2 김민선, "신령버섯 생물전환 추출물이 인간 섬유아세포의 콜라겐 합성 및 Matrix Metalloproteinase-1 생성에 미치는 영향" 한국생물공학회 34 (34): 49-53, 2019

3 J. Krutmann, "The skin aging exposome" 85 : 152-161, 2017

4 I. M. Sussex, "The scientific roots of modern plant biotechnology" 20 : 1189-, 2008

5 T. Pillaiyar, "Skin whitening agents: medicinal chemistry perspective of tyrosinase inhibitors" 32 : 403-425, 2017

6 L. Wang, "Simultaneous determination of imperatorin and its 2 metabolites in dog plasma by using liquid chromatography–tandem mass spectrometry" 70 : 640-646, 2012

7 L. C. Magdalena, "Reactive oxygen species, cellular redox systems, and apoptosis" 48 (48): 749-762, 2010

8 M. Schieber, "ROS Function in Redox Signaling and Oxidative Stress" 24 (24): 453-462, 2014

9 T. Holland, "Putting the spotlight back on plant suspension cultures" 7 : 297-, 2016

10 류지영, "Prunin의 자외선 B에 의해 손상된 인간 각질형성세포 (HaCaT)에 대한 광노화 억제 효과" 한국피부과학연구원 17 (17): 139-147, 2019

11 Marisol Ochoa-Villarreal, "Plant cell culture strategies for the production of natural products" 생화학분자생물학회 49 (49): 149-158, 2016

12 M. Ikeuchi, "Plant callus: mechanisms of induction and repression" 25 : 3159-, 2013

13 B. Ni, "Phytochemical and Biological Properties of Ajuga decumbens (Labiatae): A Review" 14 (14): 1525-1536, 2015

14 I. Liguori, "Oxidative stress, aging, and diseases" 13 : 757-772, 2018

15 M. Rinnerthaler, "Oxidative Stress in Aging Human Skin" 5 : 545-589, 2015

16 L. Denat, "Melanocytes as Instigators and Victims of Oxidative Stress" 134 : 1512-1518, 2014

17 M. A. Farage, "Intrinsic and extrinsic factors in skin ageing: a review" 30 : 87-95, 2008

18 P. M. Michael, "How mitochondria produce reactive oxygen species" 417 (417): 1-13, 2009

19 D. Pimentel, "Global warming, population growth, andnatural resources for food production" 4 : 347-, 1991

20 B. Poljšak, "Free radicals and extrinsic skin aging" 2012

21 이기복, "Evaluation of Alcea rosea L. Callus Extract as a Natural Cosmetic Ingredient" 사단법인 대한화장품학회 44 (44): 295-302, 2018

22 최해리, "Enhancing the antimicrobial effect of genistein by biotransformation in microbial system" 한국공업화학회 63 : 255-261, 2018

23 L. Q. Zhang, "Effects of β-glucosidase hydrolyzed products of harpagide and harpagoside on cyclooxygenase-2(COX-2) in vitro" 19 : 4882-4886, 2011

24 W. P. Bowe, "Cosmetic benefits of natural ingredients" 13 (13): 1021-1025, 2014

25 M. Takasaki, "Cancer chemopreventive agents (antitumor-promoters) from Ajuga decumbens" 62 : 972-975, 1999

26 Y. Ono, "Beneficial effects of Ajuga decumbens on osteoporosis and arthritis" 31 : 1199-1204, 2008

27 심중현, "B16F10 세포에서 브라질린의 미백 효능" 한국피부과학연구원 17 (17): 287-294, 2019

28 T. Murashige, "A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures" 15 (15): 473-497, 1962