In TetR-VP16-mediated transcription activation stystem TetR-VP16 hobodimer activates the tet operator modified minimal promoter. To determine the effect of the heterodimer of TetR-VP16 on transcriptional activation from that minimal promoter cotransfe...
In TetR-VP16-mediated transcription activation stystem TetR-VP16 hobodimer activates the tet operator modified minimal promoter. To determine the effect of the heterodimer of TetR-VP16 on transcriptional activation from that minimal promoter cotransfection assays were performed with U373 cells. In coexpression of TetR-VP16 and TetR the expression from the minimal promoter was activated 4-12 fold higher than in expression of TetR-VP16, suggesting that the usage of the heterodimer of TetR-VP16 can improve the effecacy of TetR-VP16-mediated transcription activation system