PPARδ의 활성 또는 과발현은 PGC-1α 단백질의 분해를 지연시킨다 = PPARδ activation or overexpression attenuate PGC-1α protein degradation

다국어 초록 (Multilingual Abstract)

PURPOSE: The purpose of this study was to evaluate whether PPARδattenuates PGC-1α protein degradation in mouse skeletal muscle after a single bout of swimming exercise (Ex). METHODS: PPARδactivation in mouse skeletal muscle was achieved by high fat diet (HFD) for 2 weeks. PPARδoverexpression in mouse skeletal muscle was achieved by electroporation (EPO). To evaluate PGC-1α, cytochrome c oxidase subunit Ⅳ (COXⅣ) and succinate- ubiquinone oxidoreductase (SUO) protein in mouse skeletal muscle, tibialis anterior (TA) muscle were dissected at 24 h or 54 h after a single bout of swimming exercise. RESULTS: PGC-1α and mitochondrial enzymes in all exercised muscle were significantly increased by a single bout of swimming exercise when compared to non-exercised muscle. PGC-1α expression in 54 h post-Ex Chow group was decreased 60% when compared to 24 h post-Ex Chow group, but 54 h post-Ex HFD group was not significantly decrease when compared to 24 h post-Ex HFD. PGC-1αexpression in 54 h post-Ex HFD group was higher 1.4 fold than 54 h post-Ex Chow group. PGC-1α expression in 54 h post-Ex EV group was decreased 75% when compared to 24 h post-Ex Ev group, but 54 h post-Ex PPARδ group was decreased 1% when compare to 24 h post-Ex PPARδ. PGC-1α expression in 54 h post-Ex PPARδ group was 1.9 fold higher than 54 h post-Ex EV group. COXⅣ와 SUO expression in 54 h post-Ex PPARδ group were 1.4 and 1.94 fold higher than 54 h post-Ex EV group. CONCLUSIONS: This study demonstrated that PPARδ activation or overexpression attenuates PGC-1α protein degradation in skeletal muscle after a single bout of swimming exercise that causes a more increase mitochondrial biogenesis.

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