An Oligonucleotide Microarray Bait for Isolation of Target Gene Fragments = An Oligonucleotide Microarray Bait for Isolation of Target Gene Fragments

다국어 초록 (Multilingual Abstract)

A new molecular-baiting method was studied by retrieving targeted gene fragments from an oligonucleotide microarray bait after hybridization. To make the microarray bait, 70-mer oligonucleotides that were designed to specifically represent the SSAl gene of Saccharomyces cerevisiae were printed on the slide. Samples of the Saccharomyces cerevisiae mRNA were extracted and labeled by the RD-PCR (Restriction Display PCR) method using the CyS-labelled universal primer, then applied for hybridization. The sample fragments that hybridized to the microarray were stripped, and the eluted cDNAs were retrieved and cloned into the pMD 18-T vector for transformation, plasmid preparation, and sequencing. BLAST searching of the GenBank database identified the retrieved fragments as being identical to the SSAl gene (from 2057-2541bp). A new method is being established that can retrieve the sample fragments using an oligo-microarray-bait.