추출조건이 노니 부정근에 함유된 rubiadin의 추출 효율에 미치는 영향

다국어 초록 (Multilingual Abstract)

Rubiadin, a major compound of noni (Morinda citrifolia) adventitious root, is highly valued in pharmaceutical industry due to hepatoprotective activity. To dissect rubiadin’s effective extraction condition, extraction process of noni adventitious roots was performed with different solvent types, ratio of water to methanol (water, 20, 40, 60, 80, and 100% of methanol), extraction time, and extraction method. In contrast, we also developed a reverse- phase HPLC assay method to determine rubiadin from noni adventitious roots. The HPLC assay of rubiadin was performed by C-18 column using a gradient solvent system of methanol and water with UV detector at 280 ㎚. The extraction efficiency of different types of solvents were increased in order of methanol (0.08%)＞ethanol (0.05%)＞acetonitrile (0.03%)＞acetone (0.02%) and methylene chloride (0.02%). The results of rubiadin extraction using different solvents showed that 1 hour of ultrasonic extraction was effective in order of 60% methanol (0.21%)＞80% methanol (0.13%)＞100% methanol (0.07%), 40% methanol (0.07%) and 2 hours of reflux extraction was effective in order of 60% methanol (0.21%)＞40% methanol (0.17%)＞80% methanol (0.14%). To compare the extraction efficiency of rubiadin according to the extraction methods and time for high rubiadin content, the extracts of rubiadin in noni adventitious roots were isolated with the methods of ultrasonic extraction, shaking extraction and reflux extraction. Rubiadin extracted from the methods of ultrasonic waves and shaking displayed the highest contents at 8 and 24 hours, respectively.

참고문헌 (Reference)

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