Isolation of Hepatoprotective Phenylpropanoid from Lactuca indica

다국어 초록 (Multilingual Abstract)

In continuing our search for biologically active compounds from Korean Compositae medicinal plants, we investigated the constituents of the aerial parts of Lactuca indica L. and isolated a phenylpropanoid derivative from its MeOH extract. The chemical structure was characterized by spectroscopic methods, including 1D and 2D NMR to be di-E-caffeoyl-meso-tartaric acid (1). Compound 1 was isolated for the first time from this plant. In this paper, we suggest that the NMR assignment at C-2 of (+)-taraxafolin-B should be corrected. In the human HBV-transfected liver cell line HepG2.2.15, the compound 1 effectively reduced HBV DNA level in the release of mature HBV particles from HepG2.2.15 cultivation.

참고문헌 (Reference)

1 Korba, B.E, "Use of a standardized cell culture assay to assess activities of nucleoside analogs against hepatitis B virus replication" 19 : 55-70, 1992

2 Hui, W.H, "Triterpenoid and steroid constituents of some Latuca and Ageratum species of Hong Kong" 10 : 899-901, 1971

3 Bergmana, M, "The antioxidant activity of aqueous spinach extract: chemical identification of active fractions" 58 : 143-152, 2001

4 김기현, "Terpene and Phenolic Constituents of Lactuca indica L." 대한약학회 31 (31): 983-988, 2008

5 Fan, M, "Study on chemical constituents from Lactuca indica" 29 : 1146-1148, 2004

6 Kan, W.S, "Pharmaceutical Botany, 7th ed"

7 Kim, K.H, "Neolignans from Piper kadsura and their anti-neuroinflammatory activity" 20 : 409-412, 2010

8 Kim, K.H, "Isolation of quinic acid derivatives and flavonoids from the aerial parts of Lactuca indica L. and their hepatoprotective activity in vitro" 17 : 6739-6743, 2007

9 Makoto, K, "Identification of Flavones in Sixteen Compositae Species" 42 : 475-477, 1978

10 Stephen, J.P, "High-resolution 1H- and 13CNMR. Spectra of D-glucopyranose, 2-acetamido-2-deoxy-Dglucopyranose, and related compounds in aqueous media" 59 : 19-34, 1977

11 Yang, J, "Differentially expressed cellular genes following HBV: Potential targets of anti- HBV drugs?" 12 : 357-363, 2005

12 Veit, M, "Di-E-caffeoylmeso- tartaric acid in the barren sprouts of Equisetum arvense" 30 : 527-529, 1991

13 Yann, L.L, "Chemical constituents from root of Taraxacum formosanum" 53 : 853-855, 2005

14 윤정은, "Antiviral Potential of the Silkworm Deoxynojirimycin against Hepatitis B Virus" 한국잠사학회 7 (7): 139-144, 2003

15 Kusamran, W.R, "Antimutagenic and anticarcinogenic potentials of some Thai vegetables" 402 : 247-258, 1998

16 Hata, K, "Activators of melanin biosynthesis from Lactuca indica" 57 : 238-241, 2003