Nerve Cell Differentiation Using Constant and Programmed Electrical Stimulation through Conductive Non-functional Graphene Nanosheets Film

다국어 초록 (Multilingual Abstract)

Electrical signal is one of the most important elctrophysiological phenomena and is widely used in electrogensisand electrodiagnosis. The use of appropriate external electrical stimulation (ES) to in situ modify cellularbehavior can desirably promote tissue regeneration. Considering its high electron mobility and surface area,graphene could become a promising conductive scaffold in biomedical applications. In this study, a programmed ES,comprehensively concerning the ES factors, such as ES period, intensity, frequency, electrical pulse and intervalchange, was used to modify Rat pheochromocytoma PC-12 cells behaviours through a large size non-functionalgraphene nano-film (NGNF) which was prepared by spray coating the high conducting graphene sheets onto polyurethanefilm. The constant ES was used to select the optimized ES intensity and as well as control. The optimizedES condition with intensity of 100 mV/mm was shown to significantly enhance PC-12 cell differentiation, neuriteextension and growth. Comparing the results by different ES, i.e. the constant 100 mV/mm and the programmed ES100 mV/mm at 1 Hz and 10 Hz, the programmed ES increased longer neuritis length. The positive enhancement tonerve behaviour by programmed ES was still significant maintained at long periods of ES (48 h).

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