CABYV 감염 멜론의 황화증상에 따른 생리적인 특성

다국어 초록 (Multilingual Abstract)

Melon leaves showing yellowing symptoms were analyzed using electron microscopy and RT-PCR for major cucurbit-infecting-viruses (CMV, MNSV, CGMMV, SqMV, WMV, KGMMV, PRSV and ZYMV) reported in Korea, but these viruses were not detected. As the result of further analysis by next-generation sequencing (NGS), the virus was identified as Cucurbit aphid-borne yellows virus (CABYV), and then confirmed by RT-PCR using CABYV-specific primers. When photosynthetic capacity was measured based on chlorophyll fluorescence yield (ChlFY), the leaves of the diseased plants showed 4.09 μ㏖?m<SUP>-2</SUP>?s<SUP>-1</SUP>, which was one-third of the readings observed for unaffected normal plants (12.36 μ㏖?m<SUP>-2</SUP>?s<SUP>-1</SUP>). The root functions of plants affected by leaf yellowing symptoms (LYS) was 0.28 ㎎?g<SUP>-1</SUP>, about half that measured for the normal unaffected plants (0.48 ㎎?g<SUP>-1</SUP>). Cytological observations revealed that there were no morphological differences in the palisade parenchyma and mesophyll spongy cells of the leaves between the diseased and the normal plants. However, the same leaf cells of the affected plants contained more starch granules compared to those of the normal, unaffected plants. We conclude that the LYS of muskmelon is not merely a physiological disorder but a viral disease caused by CABYV and spread by aphids.

참고문헌 (Reference)

1 Takeshita, S., "Vegetable gardening encyclopedia" Rural Culture Association Japan 375-379, 2004

2 Li, H., "The sequence alignment/map format and SAM tools" 25 : 2078-2079, 2009

3 Tan, S. H., "The genome structure of kyuri green mottle mosaic tobamovirus and its comparison with that of cucumber green mottle mosaic tobamovirus" 145 : 1067-1079, 2000

4 Lecoq, H., "The Luteoviridae" CAB International 1999

5 McKenna, A., "The Genome Analysis Toolkit : a Map reduce framework for analyzing next-generation DNA sequencing data" 20 : 1297-1303, 2010

6 Rajamony, L., "Resistance to Cucumber Green Mottle Mosaic Virus(CGMMV)in Muskmelon" 10 : 58-59, 1987

7 Vucurovic, A., "Presence and distribution of oilseed pumpkin viruses and molecular detection of Zucchini yellow mosaic virus" 24 : 85-94, 2009

8 Lecoq, H., "Potential involvement of melon fruit in the long distance dissemination of cucurbit potyviruses" 87 : 955-959, 2003

9 Epel, B. L., "Plant viruses spread by diffusion on ER-associated movement-protein-rafts through plasmodesmata gated by viral induced host β-1, 3-glucanases" 20 : 1074-1081, 2009

10 Moon, J. H., "Physiological responses of cucumber to root-zone temperature" Seoul National Univ. 2001

11 Bateson, M. F., "Papaya ringspot potyvirus : isolate variability and the origin of PRSV type P(Australia)" 75 : 3547-3553, 1994

12 Choi, G. S., "Occurrence of two tobamovirus diseases in cucurbits and control measures in Korea" 17 : 243-248, 2001

13 Avgelis, A. D., "Occurrence of squash mosaic virus in melons in Greece" 38 : 111-113, 1989

14 Dahal, G., "Occurrence of papaya ringspot potyvirus and cucurbit viruses in Nepal" 130 : 491-502, 1997

15 Grafton-Cardwell, E. E., "Occurrence of mosaic viruses in melons in the central valley of California" 80 : 1092-1097, 1996

16 Guilley, H., "Nucleotide sequence of Cucurbit aphid-borne yellows luteovirus" 202 : 1012-1017, 1994

17 Lee, J. Y., "New and old roles of plasmodesmata immunity and parallels to tunneling nanotubes" 221-222 : 13-20, 2014

18 NIAST., "Methods of soil chemical analysis. National Institute of Agricultural Science and Technology" RDA 2000

19 Tamura, K., "MEGA6 : molecular evolutionary genetics analysis version 6. 0" 30 : 2725-2729, 2013

20 Ryu, S. H., "List of Plant Diseases in Korea"

21 Diaz-Pendon, J. A., "Inheritance of resistance to Watermelon mosaic virus in Cucumis melo that impairs virus accumulation, symptom expression, and aphid transmission" 95 : 840-846, 2005

22 Papayiannis, L. C., "Incidence of viruses infecting cucurbits in Cyprus" 153 : 530-535, 2005

23 Tomassoli L., "First report of cucurbit aphidborne yellows virus in Italy" 56 : 720-, 2007

24 Abou-Jawdah, Y., "First report of cucurbit aphid-borne yellows luteovirus in Lebanon" 81 : 1331-, 1997

25 Lemaire, O., "First report of Cucurbit aphid-borne yellows virus in the United States" 77 : 1169-, 1993

26 Mnari, H. M., "First report of Cucurbit aphid-borne yellows virus in Tunisia causing yellows on five cucurbitaceous species" 89 : 776-, 2005

27 Juarez, M., "First report of Cucurbit aphid-borne yellows virus in Spain" 88 : 907-, 2004

28 Langmead, B., "Fast gapped-read alignment with Bowtie 2" 9 : 357-359, 2012

29 Park D. K., "Effects of leaf chlorosis on the melon fruits and growing" 29 : 66-67, 2011

30 이주현, "Effect of Different Rootstocks on Wilting Occurrence, Plant Growth, and Fruit Quality of Melon" 한국원예학회 27 (27): 211-217, 2009

31 Omar, A. F., "Cucurbit aphid-borne yellows virus in Egypt" 40 : 177-184, 2012

32 Chang, S. C., "Compounding of Luft’s epon embedding medium for use in electron microscopy with reference to anhydride : Epoxide ratio adjustment" 29 : 337-342, 1973

33 Xiang, H. Y., "Complete sequence analysis reveals two distinct poleroviruses infecting cucurbits in China" 153 : 1155-1160, 2008

34 최국선, "Characterization of Melon nerotic spot virus Isolated from Muskmelon" 한국식물병리학회 19 (19): 123-127, 2003

35 Berridge, M. V., "Biotechnol. Annu. Rev" Elsevier 127-152, 2005

36 Dukic, N., "Biological and serological characterization of viruses of summer squash crops in Yugoslavia" 47 : 149-160, 2002

37 Carver, T., "BamView : viewing mapped read alignment data in the context of the reference sequence" 26 : 676-677, 2010

38 Lecoq, H., "Adv. Virus Res" Academic Press 67-126, 2012

39 Enzie, W. D., "A source of muskmelon mosaic resistance found in the oriental pickling melon, Cucumis melo var. conomon" 43 : 195-198, 1943

40 Lee, J. Y., "A plasmodesmata-localized protein mediates crosstalk between cell-to-cell communication and innate immunity in Arabidopsis" 23 : 3353-3373, 2011

41 김정수, "2007년 우리나라 주요 작물 바이러스병 발생 상황" 한국식물병리학회 14 (14): 1-9, 2008

42 김정수, "2007−2011 우리나라 농업현장 임상진단 요청 작물의 바이러스 감염 특성" 한국식물병리학회 18 (18): 277-289, 2012