돼지 분변에서 분리한 유산균의 Probiotics 활성 비교 = Comparative Evaluation of Probiotic Activities of Lactic Acid Bacteria Isolated from Swine Feces

다국어 초록 (Multilingual Abstract)

In this study, we aimed to isolate beneficial bacteria from the feces of a weaning piglet and a sow under anaerobic conditions using three culture media types: brain heart infusion containing cysteine and mucin (BHI-CM), de Man Rogosa Sharpe medium containing cysteine (MRS-C) with adjusted pH, and blood liver containing nalidixic acid, paromomycin, neomycin, and lithium chloride (BL-NPNL). In piglet fecal samples, total viable anaerobes were 6.71, 6.14, and 6.22 log CFU/g for the BHI-CM, MRS-C, and BL-NPNL media, respectively. Sow fecal samples exhibited slightly higher counts of 8.54, 7.41, and 6.15 log CFU/g for the BHI-CM, MRS-C, and BL-NPNL media, respectively. BHI-CM was the most suitable medium for isolating a diverse range of bacteria present in feces. In the MRS-C medium, we could selectively cultivate lactic acid bacteria. In contrast, we failed to isolate any Bifidobacterium species from any fecal samples using BL-NPNL, a selective medium for Bifidobacterium species in dairy products. In total, we successfully isolated three probiotic candidates: Ligilactobacillus ruminis SY1, Limosilactobacillus reuteri SY2, and Bifidobacterium boum SY611. These strains exhibited strong resistance to bile and acidic conditions and demonstrated a high adhesion capacity to epithelial cells, suggesting their potential for intestinal colonization