Normal‐phase thin‐layer chromatography (NP‐TLC) is an established method for the separation of all major phospholipid classes according to the different polarities of the head groups. In contrast, reversed‐phase (RP)‐TLC is much less frequen...
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https://www.riss.kr/link?id=O120016317
2019년
-
0951-4198
1097-0231
SCI;SCIE;SCOPUS
학술저널
60-65 [※수록면이 p5 이하이면, Review, Columns, Editor's Note, Abstract 등일 경우가 있습니다.]
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
Normal‐phase thin‐layer chromatography (NP‐TLC) is an established method for the separation of all major phospholipid classes according to the different polarities of the head groups. In contrast, reversed‐phase (RP)‐TLC is much less frequen...
Normal‐phase thin‐layer chromatography (NP‐TLC) is an established method for the separation of all major phospholipid classes according to the different polarities of the head groups. In contrast, reversed‐phase (RP)‐TLC is much less frequently used for this purpose. This study aimed to compare the NP and the RP approach regarding their separation potential of phospholipid oxidation products.
Commercially available 1‐palmitoyl‐2‐oleoyl‐sn‐phosphatidylcholine (POPC) (PC 16:0/18:1) and 1‐palmitoyl‐2‐linoleoyl‐sn‐phosphatidylcholine (PLPC) (PC 16:0/18:2) were oxidized by NaMnO4. Oxidation products were subsequently separated by NP‐ and RP‐TLC and analyzed by electrospray ionization mass spectrometry.
In comparison with NP‐TLC, RP‐TLC was clearly superior regarding the separation of oxidation products of phospholipids. RP‐TLC enabled the separation not only of primary oxidation products of POPC such as alcohols and ketones but also of secondary oxidation products. Furthermore some oxidation products, such as aldehydes, were only detectable by ESI after RP‐TLC but not after NP‐TLC.
RP‐TLC is the method of choice to characterize oxidized PL such as oxidized phosphatidylcholines.