음극아크법으로 증착된 금속질화물 박막에 대한 포유류 세포의 시험관내 반응 : In Vitro Mammalian Cell Response to Cathodic Arc Deposited Metal Nitride Thin Films

다국어 초록 (Multilingual Abstract)

Osteoblasts cell response to TiN, TiAlSiN, TiCrAlSiN, CrN, CrAlSiN and ZrAlSiN thin films and Ti thin films was evaluated in vitro. In this work, cell adhesion, actin cytoskeleton, microtubule organization and focal contact adhesion as well as cell proliferation were investigated. Cell adhesion, actin stress fibers and microtubule organization were significant difference on the (TiAlSiN, TiCrAlSiN, CrN, CrAlSiN thin films), and the Ti thin film whereas cell ashesion, actin stress fiber and microtubule organization were slightly difference on TiN thin film and Ti thin films. Immunofluorescent staining of vinculin in osteoblasts cell also showed various focal no focal adhesions were detected on the CrN thin films. The TiAlSiN, TiCrAlSiN, CrAlSiN and ZrAlSiN thin films showed significant greater cell proliferation in relation to the glass surface or Ti thin films whereas the TiN thin film displayed a slightly higher cell proliferation compared to that on glass surface or Ti thin film. Inconstrast, the CrN thin films showed significant lower cell adhesion and proliferation in relation to glass surface or Ti thin films. As a result, the TiN, TiAlSiN, TiCrAlSiN, CrAlSiN and ZrAlSiN thin film could be a potential candidate as a tribological coating to be used for supporting hard tissue on implant surfaces whereas the CrN thin film could be a promising hard thin film to be used for inhibiting tissue growth on implant surfaces.