Intratesticular Injection of Hypertonic Saline : Non-Invasive Alternative Method for Animal Castration Model

다국어 초록 (Multilingual Abstract)

Previous studies, including our own, have demonstrated that the intratesticular injection of hypertonic saline (20%) decreased serum testosterone level which was similar to the surgical castration in the rat, showing the state of chemical castration. In the present study, we further verify the efficacy of this less invasive method as an alternative of surgical orchidectomy in the andrological field. Sterilized 20% saline was directly injected into the adult male rats (750 μl per testis). The tested rats were divided into 3 groups including intact group (intact), orchidectomy group (ORX) and saline injection group (SAL) after bilateral orchidectomy was performed at the same day of injection. All rats were sacrificed at 4 weeks after injection. The reproductive organs (testes, epididymis, seminal vesicles and prostates) were collected and used for DNA and protein pattern analyses. Also, patho-histological studies on the testes were performed. In contrast to the intact group, similar DNA damages of testis and seminal vesicle were appeared in ORX group and SAL group. The DNA degradations seemed to be the results of necrosis rather than apoptosis. In the protein pattern analysis, all the testing tissues exerted similar patterns in the ORX group and the SAL group compared to the those of intact group. Patho-histological studies revealed that severe degenerative changes in testicular seminiferous tubules and massive infiltration of immune cells in SAL group. The present study confirmed that direct injection of hypertonic saline into the testis caused the equivalent biochemical changes in the accessory sex organs as shown in the orchidectomized animals. These results suggest that hypertonic saline injection model could be a useful castration model which can substitute for surgical castration when its safety is secured through further study in the future.

참고문헌 (Reference)

1 곽병국, "고장성 식염수 주사에 의한 흰쥐 정소의 해부학적 변화" 한국발생생물학회 14 (14): 281-286, 2010

2 Moul JW, "Timely diagnosis of testicular cancer" 34 (34): 109-117, 2007

3 Wiebe JP, "The control of male fertility by 1,2,3-trihydroxypropan (THP; glycerol): rapid arrest of spermatogenesis without altering libido, accessory organs, gonadal steroidogenesis and serum testosterone. LH and FSH" 29 : 291-302, 1984

4 Jana K, "Sterilization of male stray dogs with a single intratesticular injection of calcium chloride: a dose-dependent study" 75 : 390-400, 2007

5 Oliveira EC, "Permanent contraception of dogs induced with intratesticular injection of a zinc gluconate-based solution" 77 : 1056-1063, 2012

6 Kaczmarek A, "Necroptosis: the release of damage-associated molecular patterns and its physiological relevance" 38 (38): 209-223, 2013

7 Declercq W, "Necroptosis, necrosis and secondary necrosis converge on similar cellular disintegration features" 17 (17): 922-930, 2010

8 Vanden Berghe T, "Necroptosis, necrosis and secondary necrosis converge on similar cellular disintegration features" 17 (17): 922-930, 2010

9 Galluzzi L, "Necroptosis turns TNF lethal" 35 (35): 849-851, 2011

10 Jones R, "Hormonal regulation of protein synthesis in the rat epididymis. Characterization of androgen-dependent and testicular fluid-dependent proteins" 188 (188): 667-676, 1980

11 Emir L, "Hormonal and pathologic changes after chemoablation of testes with hypertonic saline solution as a treatment method alternative to orchiectomy in patients with hormone sensitive metastatic prostatic cancer" 29 : 212-217, 2011

12 Oefelein MG, "Failure to achieve castrate levels of testosterone during luteinizing hormone releasing hormone agonist therapy: the case for monitoring serum testosterone and a treatment decision algorithm" 164 (164): 726-729, 2000

13 Jana K, "Evaluation of single intratesticular injection of calcium chloride for nonsurgical sterilization in adult albino rats" 73 (73): 289-300, 2006

14 Toney TW, "Estrogen and androgen regulation of protein synthesis by the immature rabbit epididymis" 125 (125): 231-242, 1989

15 Damber JE, "Endocrine therapy for prostate cancer" 44 (44): 605-609, 2005

16 Brito LF, "Effects of intratesticular zinc gluconate treatment on testicular dimensions, echodensity, histology, sperm production, and testosterone secretion in American black bears (Ursus americanus)" 75 : 1444-1452, 2011

17 Jana K, "Dose-dependent response to an intratesticular injection of calcium chloride for induction of chemosterilization in adult albino rats" 26 (26): 651-673, 2002

18 Steinberg M, "Degarelix: a gonadotropin-releasing hormone antagonist for the management of prostate cancer" 31 (31): 2312-2331, 2009

19 Nikoletopoulou V, "Crosstalk between apoptosis, necrosis and autophagy" 1833 (1833): 3448-3459, 2013

20 Emir L, "Chemical castration with intratesticular injection of 20% hypertonic saline: a minimally invasive method" 26 (26): 392-396, 2008

21 Krysko DV, "Apoptosis and necrosis: detection, discrimination and phagocytosis" 44 (44): 205-221, 2008

22 D'Agostino A, "Androgenic regulation of messenger RNA in rat epididymis" 190 (190): 505-512, 1980