COVID-19 진단기법: Primer에 따른 SARS-CoV-2 (COVID-19)의 핵산기반 진단법 분석

다국어 초록 (Multilingual Abstract)

Since the first outbreak in Wuhan, China, Coronavirus Disease 2019 (COVID-19) has spread rapidly around the world and there are still many confirmed cases worldwide. In this serious situation, the rapid diagnosis and classification of patients who are infected with COVID-19 are very important to individuals and society. There are two main methods of testing in COVID-19: nucleic acid testing and immunological testing, but the test currently used in the confirmatory assay is nucleic acid testing, due to its accuracy. This nucleic acid test uses RT-PCR which is using reverse transcriptase, because SARS-CoV-2 is RNA virus. One of the important factors determining accuracy in RT-PCR is the primer sequences. In other words, it is very important for the accuracy of the test that it adheres exactly to the target gene. Therefore, this review focused on primer and described the test methods for SARS-CoV-2 detection. The main targets of the primers in SARS-CoV-2 were ORF1ab/RdRp gene, E gene, N gene, and S gene. I conducted comparisons of several primer probe sets of these target genes, and also checked the primer configuration of several commercial diagnostic kits to inspect the actual utilization. As a result, most of the genes I mentioned were frequently used as targets, and most of them were highly reliable.

목차 (Table of Contents)

• Abstract
• 1. INTRODUCTION
• 2. MATERIALS AND METHODS
• 3. CONCLUSION
• REFERENCES

참고문헌 (Reference)

1 Alcoba-Florez, J., "Sensitivity of different RTqPCR solutions for SARS-CoV-2 detection" 99 : 190-192, 2020

2 김종식, "SARS-CoV-2의 진단기술" 한국생명과학회 30 (30): 731-741, 2020

3 Yang, Y., "SARS-CoV-2 : characteristics and current advances in research" 17 : 117-, 2020

4 Bustin, S. A., "RT-qPCR testing of SARSCoV-2: a primer" 21 : 3004-, 2020

5 Li, D., "Primer design for quantitative real-time PCR for the emerging coronavirus SARS-CoV-2" 10 : 7150-7162, 2020

6 Perkinelme, "PerkinElmer® SARS-CoV-2 Nucleic acid detection kit (RUO)"

7 Toptan, T., "Optimized qRT-PCR approach for the detection of intraand extra-cellular SARS-CoV-2 RNAs" 21 : 4396-, 2020

8 Myungsun Park, "Optimization of primer sets and detection protocols for SARS-CoV-2 of coronavirus disease 2019 (COVID-19) using PCR and real-time PCR" 생화학분자생물학회 52 : 1-15, 2020

9 Peñarrubia, L., "Multiple assays in a real-time RTPCR SARS-CoV-2 panel can mitigate the risk of loss of sensitivity by new genomic variants during the COVID-19 outbreak" 97 : 225-229, 2020

10 Mousavizadeh, L., "Genotype and phenotype of COVID-19 : Their roles in pathogenesis" 31 : 2020

11 Khailany, R. A., "Genomic characterization of a novel SARS-CoV-2" 19 : 100682-, 2020

12 Visseaux, B., "Evaluation of the QIAstat-Dx respiratory SARS-CoV2 panel, the first rapid multiplex PCR commercial assay for SARS-CoV-2 detection" 58 : e00630-e00620, 2020

13 Hur, K. -H., "Evaluation of four commercial kits for SARS-CoV-2 real-time reverse-transcription polymerase chain reaction approved by emergency-use-authorization in Korea" 7 : 521-, 2020

14 Petrovan, V., "Evaluation of commercial qPCR kits for detection of SARS-CoV-2 in pooled samples" 10 : 472-, 2020

15 Corman, V. M., "Detection of 2019 novel coronavirus(2019-nCoV)by real-time RT-PCR" 25 : 2000045-, 2020

16 Lu, Y., "Comparison of the diagnostic efficacy between two PCR test kits for SARS-CoV2 nucleic acid detection" 34 : e23554-, 2020

17 Van Kasteren, P. B., "Comparison of seven commercial RT-PCR diagnostic kits for COVID-19" 128 : 104412-, 2020

18 Iglói, Z., "Comparison of commercial realtime reverse transcription PCR assays for the detection of SARS-CoV-2" 129 : 104510-, 2020

19 Mollaei, H. R., "Comparison five primer sets from different genome region of COVID-19 for detection of virus infection by conventional RT-PCR" 12 : 185-193, 2020

20 Nalla, A. K., "Comparative performance of SARS-CoV-2detection assays using seven different primer-probe sets and one assay kit" 58 (58): e00557-e00520, 2020

21 Jung, Y. J., "Comparative analysis of primer-probe sets for the laboratory confirmation of SARS-CoV-2" 2513-2523, 2020

22 김영진, "COVID-19 Testing in South Korea: Current Status and the Need for Faster Diagnostics" 대한진단검사의학회 40 (40): 349-350, 2020

23 BioCore, "BioCore 2019-nCoV real time PCR kit manual"

24 Vogels, C. B. F., "Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets" 5 : 1299-1305, 2020