Rapid induction of edible lipids in <i>Chlorella</i> by mild electric stimulation

다국어 초록 (Multilingual Abstract)

<P><B>Abstract</B></P> <P>In this work, a new stress-based method for rapid induction of triacylglycerol (TAG) and total and polyunsaturated fatty acid accumulations in <I>Chlorella</I> sp. by mild electric stimulation is presented. When a cathodic current of 31 mA (voltage: 4 V) was applied to the algal cells for 4 h, the TAG content of the electro-treated cells was sharply increased to a level 2.1 times that of the untreated control. The contents of the polyunsaturated linoleic (C18:2n6) and linolenic (C18:3n3) acids in the electro-treated cells were also 36 and 57% higher than those in the untreated cells, respectively. Cyclic voltammetry and various biochemical analyses indicate that TAG and fatty acid formations are electro-stimulated via <I>de novo</I> fatty acid biosynthesis and metabolic transformation in the <I>Chlorella</I> cells.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Rapid induction of lipids in <I>Chlorella</I> by mild electric treatment (31 mA and 4 h). </LI> <LI> 2.1-fold improvement of triacylglycerol (TAG) content relative to the control. </LI> <LI> Promotion of biosynthesis of polyunsaturated linoleic and linolenic acids. </LI> <LI> Electric lipid stimulation through transformation and <I>de novo</I> biosynthesis. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>