Double-layer hybrid nanofiber membranes by electrospinning with sustained antibacterial activity for wound healing

다국어 초록 (Multilingual Abstract)

Bacterial infection is a general problem that increases the burden of wound treatment. It has been intenselyexpected to fabricate bioactive wound dressings with good antibacterial effect. In this work, doublelayerhybrid nanofiber membranes (DHNM) of PVA/COL@ZnONPs and PCL/Gt@TiO2NPs were built by continuouselectrospinning. The matrix membranes with acceptable tensile strength and moisture permeabilityproperty were obtained by optimizing the formulation and spinning conditions. The matrixmembranes exhibited Young’s modulus of 91.93 MPa and tensile stress of 2.61 MPa. The water vaportransmittance rate (WVTR) of matrix membranes was 1140.45 g/m2day. ZnONPs in the inner layer werereleased quickly in 2 hours to destroy the bacteria in the wound. TiO2NPs in the outer layer were sustainablyreleased within 7 days to resist the bacterial attack outsides. The double-layer membranes showedgood biocompatibility and enhanced the migration of cells. The cell migration rate of double-layer hybridmembranes (DHNM) was higher than that of single layer membranes. The double-layer membraneshowed 93% elimination of the wound specific P. aeruginosa, over 99% inhibition to E. coli and over 97%to S. aureus. This study provides a potential approach for wound dressings to improve antibacterial effect.