Genetics and role of respiratory system for hydrogen sulfide production in saccharomyces cerevisiae

다국어 초록 (Multilingual Abstract)

H2S is known as an important factor for caloric restriction benefits, and it is produced through trans-sulfuration pathway in mammals. However, the detailed mechanism hasn’t been fully studied yet. In this paper, we verified H2S-producing mechanism and the relationship between H2S and chronological lifespan (CLS) in Saccharomyces cerevisiae. By using electron transport chain KO mutants and rho0, and by treating respiration inhibiting chemical (antimycin A), we found that respiration was the main pathway to produce H2S in budding yeast. Furthermore, it was tested to find out the relationship between H2S and CLS, and the relationship varied according to growth media. H2S production increased in CLS-extending conditions (caloric restriction and rapamycin) in SC media but decreased in same conditions in YPD media. Lastly, by using trans-sulfuration pathway KO mutants, it was found that H2S produced through trans-sulfuration pathway was still controlled by respiration. In conclusion, respiration was the main pathway to produce H2S, and there was no relationship between H2S and CLS.

목차 (Table of Contents)

• 1) Introduction
• 2) Materials & Methods
• 1. Yeast strains and culture conditions
• 2. Measuring CLS with colony forming unit (CFU) assay
• 3. Measurement of H2S
• 4. Oxygen consumption spot assay
• 5. Construction of yeast single gene KO mutant cys4Δ
• 3) Results
• 1. CLS of respiratory strains and non-respiratory strains
• 2. Respiratory strains emit more H2S, and H2S has no
• correlation with CLS in YPD media
• 3. Respiratory strains still produce more in SC media, but
• more H2S is produced in CR and rapamycin condition
• 4. H2S decreased when respiration was inhibited by A.A
• 5. A.A could inhibit H2S production in TSP KO mutants
• 4) Discussion
• 5) Reference