Formation of Poly(ethylene glycol)-Poly(ε-caprolactone) Nanoparticles via Nanoprecipitation

다국어 초록 (Multilingual Abstract)

Size control of therapeutic carriers in drug delivery systems has become important due to its relevance to biodistribution in the human body and therapeutic efficacy. To understand the dependence of particle size on the formation condition during nanoprecipitation method, we prepared nanoparticles from biodegradable, amphiphilic block copolymers and investigated the particle size and structure of the resultant nanoparticles according to various process parameters. We synthesized monomethoxy poly(ethylene glycol)-poly(ε-caprolactone) block copolymer, MPEG-PCL, with different MPEG/PCL ratios via ring opening polymerization initiated from the hydroxyl end group of MPEG. Using various formulations with systematic change of the block ratio of MPEG and PCL, solvent choice, and concentration of organic phase, MPEG-PCL nanoparticles were prepared through nanoprecipitation technique. The results indicated that (ⅰ) the nanoparticles have a dual structure with an MPEG shell and a PCL core,
originating from self-assembly of MPEG-PCL copolymer in aqueous condition, and (ⅱ) the size of nanoparticles is dependent upon two sequential processes: diffusion between the organic and aqueous phases and solidification of the polymer.

참고문헌 (Reference)

1 H. Pihlajamaki, 39 : 932 -, 2006

2 Z. Gatmaitan, 148 : 2027 -, 1996

3 Q. Huang, 8 : 469 -, 2002

4 R. C. Edwards, 59 : 19 -, 2001

5 H. Yoshimoto, 24 : 2077 -, 2003

6 Y. Z. Zhang, 16 : 3406 -, 2004

7 V. J. Chen, 27 : 3973 -, 2006

8 H. W. Ouyang, 75 : 264 -, 2005

9 W. J. Li, 60 : 613 -, 2002

10 Y. K. Luu, 89 : 341 -, 2003

11 C. T. Lee, 7 : 2200 -, 2006

12 R. T. Liggins, 222 : 19 -, 2001

13 F. X. Hu, 27 : 2006-, 5725

14 J. Cheng, 28 : 869 -, 2007

15 T. Govender, 57 : 171 -, 1999

16 H. Zhang, 329 : 122 -, 2007

17 X. B. Xiong, 8 : 874 -, 2007

18 C. Allen, 9 : 564 -, 1998

19 G. Z. Zhu, 18 : 52 -, 2000

20 M. Lee, 28 : 1862 -, 2007

21 E. Walter, J. Control. Release : 361 -, 1999

22 H. Cohen, 7 : 1896 -, 2000

23 X. D. Yuan, 6 : 2821 -, 2006

24 S. Galindo-Rodriguez, 21 : 1428 -, 2004

25 S. R. Bhattarai, 25 : 2595 -, 2004

26 Y. C. Dong, 25 : 2843 -, 2004

27 O. Laitinen, 61 : 33 -, 33

28 C. Deng, 7 : 590 -, 2006

29 W. Lu, 107 : 428 -, 2005

30 S. C. Lee, 89 : 437 -, 2003

31 P. Ping, 6 : 587 -, 2005

32 J. S. Lee, 6 : 1906 -, 2005

33 Z. W. Ma, 26 : 1253 -, 2005

34 S. Barbault-Foucher, 83 : 365 -, 2002

35 S. H. Oh, 24 : 4011 -, 2003

36 F. L. Mi, 23 : 3257 -, 2002

37 C. X. Song, 43 : 197 -, 1997

38 J. C. Leroux, 39 : 339 -, 1996

39 R. Gref, 263 : 1600 -, 1994

40 R. L. Juliano, 2 : 31 -, 1988

41 V. P. Torchilin, 16 : 141 -, 1995

42 S. M. Moghimi, 53 : 283 -, 2001

43 G. Storm, 17 : 31 -, 1995

44 심우선, "pH-Induced Micellization of Biodegradable Block Copolymers Containing Sulfamethazine" 한국고분자학회 13 (13): 344-351, 2005

45 K. L. Hoy, "The Hoy: Tables of Solubility Parameters" Union Carbide Corporation 1985

46 유재호, "Synthesis and Microphase Separation of Biodegradable Poly(ε-caprolactone)-Poly(ethylene glycol)-Poly(ε-caprolactone) Multiblock Copolymer Films" 한국고분자학회 16 (16): 609-613, 2008

47 Su Jin Im, "Synthesis and Characterization of Biodegradable Elastic Hydrogels Based on Poly(ethylene glycol) and Poly(ε-caprolactone) Blocks" 한국고분자학회 15 (15): 363-369, 2007

48 Changyong Choi, "Preparation and Characterization of Nanoparticles Using Poly(N-isopropylacrylamide)-Poly(ε-caprolactone) and Poly(ethylene glycol)-Poly(ε-caprolactone) Block Copolymers with Thermosensitive Function" 한국고분자학회 15 (15): 623-632, 2007

49 Hye Yun Lee, "Poly(vinyl pyrrolidone) Conjugated Lipid System for the Hydrophobic Drug Delivery" 한국고분자학회 15 (15): 547-552, 2007

50 Chiyoung Park, "Metal Nanoparticles in the Template of Poly(2-ethyl-2-oxazoline)-block-Poly(ε-caprolactone) Micelle" 한국고분자학회 15 (15): 39-43, 2007

51 C. M. Hansen, "Hansen Solubility Parameters: A User's Handbook" CRC Press 2000

52 So Yeon Kim, "Biotin-Conjugated Block Copolymeric Nanoparticles as Tumor-Targeted Drug Delivery Systems" 한국고분자학회 15 (15): 646-655, 2007