국내 육종 사과 품종인 ‘홍로’의 과피와 과육에서 발현되는 단백질체를 추출하여 이차원 전기영동으로 분리하였다. 그 결과 대부분의 단백질들은 과피와 과육에서 유사한 정도로 발현되...
http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
https://www.riss.kr/link?id=A107367538
2021
Korean
KCI등재,SCIE,SCOPUS
학술저널
191-203(13쪽)
0
0
상세조회0
다운로드국문 초록 (Abstract)
국내 육종 사과 품종인 ‘홍로’의 과피와 과육에서 발현되는 단백질체를 추출하여 이차원 전기영동으로 분리하였다. 그 결과 대부분의 단백질들은 과피와 과육에서 유사한 정도로 발현되...
국내 육종 사과 품종인 ‘홍로’의 과피와 과육에서 발현되는 단백질체를 추출하여 이차원 전기영동으로 분리하였다. 그 결과 대부분의 단백질들은 과피와 과육에서 유사한 정도로 발현되었으나, 30개의 단백질 spot들은 과피 또는 과육에서의 발현 정도가 2배 이상 차이가 났다. 이들 중 25개는 과피에서, 5개는 과육에서 더 높게 발현되었다. 이들 단백질들을 MALDI-TOF/TOF로 분석한 결과, 과피에서 더 높게 발현된 단백질 spot들 중 18개는 각각의 기능을 갖는 단백질로 동정 되었고, 나머지 7개는 기능이 밝혀지지 않은 것으로 동정되었거나 미동정 되었다. 과육에서 더 높게 발현된 단백질 spot들의 경우 2개는 각각의 기능을 갖는 단백질로 동정 되었고, 나머지 3개는 미동정 되었다. 과피에서 더 높게 발현된 단백질은 에틸렌의 생합성과 관련된 1-aminocyclopropane-1-carboxylate oxidase homolog(spot 21번), 이차대사산물의 생합성과 관련된 acetyl-CoA acetyltransferase, cytosolic 1(spot 148), acetyl-CoA acetyltransferase, cytosolic 1-like(spot 316) 및 8-hydroxygeraniol dehydrogenase-like(spot 348), 광합성과 관련된 oxygen-evolving enhancer protein 1, chloroplastic(spot 210) 및 ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit(spot 273), 생체방어 및 스트레스 반응과 관련된 (+)-neomenthol dehydrogenase-like(spot 216 및 219), formate dehydrogenase, mitochondrial(spot 305), glutathione S-transferase-like(spot 483 및 484) 및 acidic endochitinase SE 2-like, partial(spot 391 및 392), 항산화효소인 L-ascorbate peroxidase 2(spot 221), 알레르기 관련 단백질인 MLP-like protein 34(spot 406), major allergen Mal d 1(spot 451 및 452) 및 major allergen Mal d 1.06A01(spot 457)이었다. 한편 과육에서 더 높게 발현된 단백질은 protein folding과 관련된 chaperonin 60 subunit alpha 1, chloroplastic(spot 8) 및 탄수화물 대사와 관련된 NADP-dependent D-sorbitol-6-phosphate dehydrogenase(spot 380)이었다. 이상의 연구 결과들은 사과의 육종, 저장, 품질, 환경에 대한 내성 등과 관련된 광범위한 연구에 유용하게 이용될 수 있을 것으로 사료되었다.
다국어 초록 (Multilingual Abstract)
The proteome expressed in the peel and flesh of ‘Hongro’, a domestic breeding apple cultivar, was extracted and separated by two-dimensional electrophoresis. Most of the proteins were expressed at similar levels in the peel and flesh, but 30 prote...
The proteome expressed in the peel and flesh of ‘Hongro’, a domestic breeding apple cultivar, was extracted and separated by two-dimensional electrophoresis. Most of the proteins were expressed at similar levels in the peel and flesh, but 30 protein spots showed a difference of more than two times higher in expression level in the peel and flesh. Of these, 25 were higher in the peel and 5 were higher in the flesh. By analyzing these proteins with MALDI-TOF/TOF, 18 of the protein spots that were higher in the peel were identified as proteins with their respective functions, and the remaining 7 were identified as having unknown function or were unidentified. In the case of the protein spots expressed higher in the flesh, two were identified as proteins having respective functions, and the other three were unidentified. Proteins more highly expressed in the peel were 1-aminocyclopropane-1-carboxylate oxidase homolog (spot 21) related to the biosynthesis of ethylene; acetyl-CoA acetyltransferase (spot 148), cytosolic 1, acetyl-CoA acetyltransferase, cytosolic 1-like (spot 316) and 8-hydroxygeraniol dehydrogenase-like (spot 348) related to the biosynthesis of secondary metabolites; oxygen-evolving enhancer protein 1, chloroplastic (spot 210), and ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit (spot 273) related to the photosynthesis;(+)-neomenthol dehydrogenase-like (spot 216 and 219), formate dehydrogenase, mitochondrial (spot 305), glutathione S-transferase-like (spot 483 and 484), and acidic endochitinase SE 2-like, partial (spot 391 and 392) related to the biological defense and stress response; and antioxidant enzyme L-ascorbate peroxidase 2 (spot 221), MLP-like protein 34 (spot 406), major allergen Mal d 1 (spot 451 and 452), and major allergen Mal d 1.06A01 (spot 457) related to allergies. On the other hand, proteins that were more highly expressed in the flesh were chaperonin 60 subunit alpha 1, chloroplastic (spot 8) related to protein folding, and NADP-dependent D-sorbitol-6-phosphate dehydrogenase (spot 380) related to carbohydrate metabolism. These results will be useful in a wide range of studies related to apple breeding, storage, quality, and environmental tolerance.
목차 (Table of Contents)
참고문헌 (Reference)
1 김재호, "사과 ‘홍로’ 과피에서 발현되는 대규모 단백질체 분석" 한국원예학회 38 (38): 795-809, 2020
2 Marco Archetti, "Unravelling the evolution of autumn colours: an interdisciplinary approach" Elsevier BV 24 (24): 166-173, 2009
3 Laetitia B. B. Martin, "There's more than one way to skin a fruit: formation and functions of fruit cuticles" Oxford University Press (OUP) 65 (65): 4639-4651, 2013
4 Helene Pühringer, "The promoter of an apple Ypr10 gene, encoding the major allergen Mal d 1, is stress- and pathogen-inducible" Elsevier BV 152 (152): 35-50, 2000
5 Riccardo Velasco, "The genome of the domesticated apple (Malus × domestica Borkh.)" Springer Science and Business Media LLC 42 (42): 833-839, 2010
6 Flávia A.P. Ribeiro, "The chemopreventive activity of apple against carcinogenesis" Ovid Technologies (Wolters Kluwer Health) 23 (23): 477-480, 2014
7 Gitte Meriläinen, "The Thiolase Reaction Mechanism: The Importance of Asn316 and His348 for Stabilizing the Enolate Intermediate of the Claisen Condensation" American Chemical Society (ACS) 48 (48): 11011-11025, 2009
8 Xiaoping Yi, "The Manganese-stabilizing Protein Is Required for Photosystem II Assembly/Stability and Photoautotrophy in Higher Plants" Elsevier BV 280 (280): 16170-16174, 2005
9 Jörg Bohlmann, "Terpenoid biomaterials" Wiley 54 (54): 656-669, 2008
10 Eugenio Aprea, "Sweet taste in apple: the role of sorbitol, individual sugars, organic acids and volatile compounds" Springer Science and Business Media LLC 7 (7): 44950-, 2017
1 김재호, "사과 ‘홍로’ 과피에서 발현되는 대규모 단백질체 분석" 한국원예학회 38 (38): 795-809, 2020
2 Marco Archetti, "Unravelling the evolution of autumn colours: an interdisciplinary approach" Elsevier BV 24 (24): 166-173, 2009
3 Laetitia B. B. Martin, "There's more than one way to skin a fruit: formation and functions of fruit cuticles" Oxford University Press (OUP) 65 (65): 4639-4651, 2013
4 Helene Pühringer, "The promoter of an apple Ypr10 gene, encoding the major allergen Mal d 1, is stress- and pathogen-inducible" Elsevier BV 152 (152): 35-50, 2000
5 Riccardo Velasco, "The genome of the domesticated apple (Malus × domestica Borkh.)" Springer Science and Business Media LLC 42 (42): 833-839, 2010
6 Flávia A.P. Ribeiro, "The chemopreventive activity of apple against carcinogenesis" Ovid Technologies (Wolters Kluwer Health) 23 (23): 477-480, 2014
7 Gitte Meriläinen, "The Thiolase Reaction Mechanism: The Importance of Asn316 and His348 for Stabilizing the Enolate Intermediate of the Claisen Condensation" American Chemical Society (ACS) 48 (48): 11011-11025, 2009
8 Xiaoping Yi, "The Manganese-stabilizing Protein Is Required for Photosystem II Assembly/Stability and Photoautotrophy in Higher Plants" Elsevier BV 280 (280): 16170-16174, 2005
9 Jörg Bohlmann, "Terpenoid biomaterials" Wiley 54 (54): 656-669, 2008
10 Eugenio Aprea, "Sweet taste in apple: the role of sorbitol, individual sugars, organic acids and volatile compounds" Springer Science and Business Media LLC 7 (7): 44950-, 2017
11 Cécile Hourton-Cabassa, "Stress Induction of Mitochondrial Formate Dehydrogenase in Potato Leaves1" Oxford University Press (OUP) 116 (116): 627-635, 1998
12 William J. Hurkman, "Solubilization of Plant Membrane Proteins for Analysis by Two-Dimensional Gel Electrophoresis" Oxford University Press (OUP) 81 (81): 802-806, 1986
13 Gianni Teo, "Silencing leaf sorbitol synthesis alters long-distance partitioning and apple fruit quality" Proceedings of the National Academy of Sciences 103 (103): 18842-18847, 2006
14 Masanori Izumi, "RBCS1A and RBCS3B, two major members within the Arabidopsis RBCS multigene family, function to yield sufficient Rubisco content for leaf photosynthetic capacity" Oxford University Press (OUP) 63 (63): 2159-2170, 2012
15 José M. Palma, "Proteomics as an approach to the understanding of the molecular physiology of fruit development and ripening" Elsevier BV 74 (74): 1230-1243, 2011
16 Mi Hee Shin, "Proteomic analysis reveals dynamic regulation of fruit ripening in response to exogenous ethylene in kiwifruit cultivars" 한국원예학회 61 (61): 93-114, 2020
17 Carmine Guarino, "Proteomic analysis of the major soluble components in Annurca apple flesh" Wiley 51 (51): 255-262, 2007
18 Guozheng Qin, "Proteomic analysis of changes in mitochondrial protein expression during fruit senescence" Wiley 9 (9): 4241-4253, 2009
19 Young Sang Kwon, "Proteomic analyses of the interaction between the plant-growth promoting rhizobacterium Paenibacillus polymyxa E681 and Arabidopsis thaliana" Wiley 16 (16): 122-135, 2016
20 Pappachan E. Kolattukudy, "Polyesters in Higher Plants" Springer Berlin Heidelberg 71 : 1-49, 2001
21 Kenji Suzuki, "Plastid chaperonin proteins Cpn60α and Cpn60β are required for plastid division in Arabidopsis thaliana" Springer Science and Business Media LLC 9 (9): 38-38, 2009
22 Davies KM, "Plant pigments and their manipulation:Annual Plant Reviews, 14" Blackwell Publishing 1-18, 2004
23 G. Salcedo, "Plant non-specific lipid transfer proteins as food and pollen allergens" Wiley 34 (34): 1336-1341, 2004
24 Stéphane Quideau, "Plant Polyphenols: Chemical Properties, Biological Activities, and Synthesis" Wiley 50 (50): 586-621, 2011
25 Lattanzio V, "Phytochemistry: Advances in Research" Research Singpost 23-67, 2006
26 Yoshinori Kanayama, "Nucleotide Sequence of a cDNA Encoding NADP-Sorbitol-6-Phosphate Dehydrogenase from Apple" Oxford University Press (OUP) 100 (100): 1607-1608, 1992
27 V O Popov, "NAD+-dependent formate dehydrogenase" Portland Press Ltd. 301 (301): 625-643, 1994
28 Kenneth T. Douglas, "Mechanism of Action of Glutathione-Dependent Enzymes" John Wiley & Sons, Inc. 59 : 103-167, 1987
29 Ana I. Sancho, "Maturity and Storage Influence on the Apple (Malus domestica) Allergen Mal d 3, a Nonspecific Lipid Transfer Protein" American Chemical Society (ACS) 54 (54): 5098-5104, 2006
30 Anita Herndl, "Mapping ofMalus domestica allergens by 2-D electrophoresis and IgE-reactivity" Wiley 28 (28): 437-448, 2007
31 Gorji Marzban, "Localisation and distribution of the major allergens in apple fruits" Elsevier BV 169 (169): 387-394, 2005
32 Jerry D. Holman, "Informatics of Protein and Posttranslational Modification Detection via Shotgun Proteomics" Humana Press 1002 : 167-179, 2013
33 Gregory A. Armstrong, "Genetics and molecular biology of carotenoid pigment biosynthesis" Wiley 10 (10): 228-237, 1996
34 Christopher M. Richards, "Genetic diversity and population structure in Malus sieversii, a wild progenitor species of domesticated apple" Springer Science and Business Media LLC 5 (5): 339-347, 2009
35 L. Alexander, "Ethylene biosynthesis and action in tomato: a model for climacteric fruit ripening" Oxford University Press (OUP) 53 (53): 2039-2055, 2002
36 Michael C. Pirrung, "Ethylene Biosynthesis from 1-Aminocyclopropanecarboxylic Acid" American Chemical Society (ACS) 32 (32): 711-718, 1999
37 Ana I. Sancho, "Effect of Postharvest Storage on the Expression of the Apple Allergen Mal d 1" American Chemical Society (ACS) 54 (54): 5917-5923, 2006
38 Sholpan Davletova, "Cytosolic Ascorbate Peroxidase 1 Is a Central Component of the Reactive Oxygen Gene Network of Arabidopsis" Oxford University Press (OUP) 17 (17): 268-281, 2005
39 F Ulrich Hartl, "Converging concepts of protein folding in vitro and in vivo" Springer Science and Business Media LLC 16 (16): 574-581, 2009
40 Jeung Joo Lee, "Comparative proteomic study between tuberous roots of light orange- and purple-fleshed sweetpotato cultivars" Elsevier BV 193-194 : 120-129, 2012
41 R. van Ree, "Clinical importance of non-specific lipid transfer proteins as food allergens" Portland Press Ltd. 30 (30): 910-913, 2002
42 Carlos Blanco, "Class I chitinases as potential panallergens involved in the latex-fruit syndrome" Elsevier BV 103 (103): 507-513, 1999
43 Haixia Yang, "Chitinase III in pomegranate seeds (Punica granatum Linn.): a high-capacity calcium-binding protein in amyloplasts" Wiley 68 (68): 765-776, 2011
44 Jetter R, "Biology of the plant cuticle" Blackwell 145-181, 2006
45 Alexander Vershinin, "Biological functions of carotenoids - diversity and evolution" Wiley 10 (10): 99-104, 1999
46 Jeanelle Boyer, "Apple phytochemicals and their health benefits" Springer Science and Business Media LLC 3 (3): 1-15, 2004
47 Claudius Marondedze, "Apple Hypanthium Firmness: New Insights from Comparative Proteomics" Springer Science and Business Media LLC 168 (168): 306-326, 2012
48 Ikeda H, "Acyclic monoterpene primary alcohol : NADP+oxidoreductase of Rauwolfia serpentina cells : the key enzyme in biosynthesis of monoterpene alcohols" 109 : 341-347, 1991
49 Ruth Finkelstein, "Abscisic Acid Synthesis and Response" BioOne 11 : e0166-, 2013
50 Elzebroek ATG, "ATG Elzebrock and K Wind, ed, Guide to cultivated plants" CAB International 25-28, 2008
51 Marion M. Bradford, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding" Elsevier BV 72 (72): 248-254, 1976
52 Qifa Zheng, "A proteomic investigation of apple fruit during ripening and in response to ethylene treatment" Elsevier BV 93 : 276-294, 2013
53 M.J. Leaver, "A piscine glutathione S-transferase which efficiently conjugates the end-products of lipid peroxidation" Elsevier BV 46 (46): 71-74, 1998
54 Hyong Woo Choi, "A Role for a Menthone Reductase in Resistance against Microbial Pathogens in Plants " Oxford University Press (OUP) 148 (148): 383-401, 2008
55 Matsui NM, "2-D Proteome Analysis Protocols" Humana Press 301-311, 1999
56 Xuetong Fan, "1-Methylcyclopropene Inhibits Apple Ripening" American Society for Horticultural Science 124 (124): 690-695, 1999
57 R. Brehler, ""Latex-fruit syndrome": frequency of cross-reacting IgE antibodies" Wiley 52 (52): 404-410, 1997
Characteristics of Carbohydrate Assimilation and Distribution in Walnut (Juglans regia L.)
학술지 이력
연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2023 | 평가예정 | 해외DB학술지평가 신청대상 (해외등재 학술지 평가) | |
2020-01-01 | 평가 | 등재학술지 유지 (해외등재 학술지 평가) | |
2017-02-08 | 학술지명변경 | 외국어명 : Korean Journal of Horticultural Science & Technology -> Horticultural Science & Technology | |
2011-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2009-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2007-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2004-01-01 | 평가 | 등재후보학술지 선정 (신규평가) | |
2003-01-01 | 평가 | 등재후보 1차 PASS (등재후보1차) | |
2002-07-01 | 평가 | 등재후보학술지 선정 (신규평가) |
학술지 인용정보
기준연도 | WOS-KCI 통합IF(2년) | KCIF(2년) | KCIF(3년) |
---|---|---|---|
2016 | 0.92 | 0.74 | 0.83 |
KCIF(4년) | KCIF(5년) | 중심성지수(3년) | 즉시성지수 |
0.77 | 0.73 | 1.115 | 0.19 |