Chlorhexidine 처리가 탈회 상아질에 미치는 영향 = Effect of chlorhexidine on the demineralized dentin

다국어 초록 (Multilingual Abstract)

This study was designed to investigate the effects of chlorhexidine(CHX) in different concentrations and application time on the demineralized dentin by means of measurement of CHX binding amount and elastic modulus combined with SEM analysis.
Dentin beams(2mm??7mm??0.9mm) were obtained from the mid-coronal dentin of extracted noncarious human third molars. Dentin beams were divided into control group and 6 experimental groups(N=10) according to the concentration and the application time of CHX. After demineralizing dentin beams in formic acid, baseline modulus of elasticity(E) of each beam was measured by three-point bending test. Dentin beams were pretreated with 0.02%, 0.2%, 2% CHX(experimental) or without CHX(control) for 1 min or 30 min and the media was analyzed for CHX binding amount by determination of absorbance. Then dentin beam were individually incubated in artificial saliva at 37?? by 6 weeks. Three-point bending test was repeatedly performed every 2 weeks and examined in FE-SEM. The results were statistically analyzed using ANOVA and Duncan󰡑s multiple comparison's test at 95% significance level.

The results were as follows;
1. 0.2% and 2% groups showed significantly higher CHX binding amount than 0.02% group(p<0.05), but there were no significant differences between 1 min and 30 min application time in all groups(p>0.05).
2. The elastic modulus of control group showed a progressive decrease over incubation time and a significant decrease after 2 weeks incubation compared with baseline data(p<0.05).
3. In all of the experimental groups, E value were significantly increased after 4, 6 weeks of incubation than after 2 weeks of incubation(p<0.05) except 0.02%-1 group(p>0.05).
4. There was a significant positive correlation between CHX binding amount and elastic modulus after 2, 4, 6 weeks of incubation.
5. Mineral deposition in to the collagen matrix was examined and Ca/P ratio was increased as CHX concentration increased.

Within limited results, pretreatment with CHX inhibited a decrease of elastic modulus in demineralized dentin over time, which was increased in proportion to CHX binding amount.

목차 (Table of Contents)

• Ⅰ. 서 론 1
• Ⅱ. 실험방법 및 재료 2
• Ⅲ. 실험성적 4
• Ⅳ. 총괄 및 고안 7
• Ⅴ. 결 론 9
• 참고문헌 9
• 영문초록 12
• 사진부도 설명 13