꽃게, Portunus trituberculatus (Miers)의 탈피 과정 중 유전자 발현패턴

다국어 초록 (Multilingual Abstract)

This study was carried out to investigated the gene expression pattern of blue crab during
molting stages. Three types (soft, medium, hard shells) of blue crabs were divided by using
digital durometer. Total RNAs were isolated using Trizol solution from the blue crabs. 14,370
EST clones in the NCBI gene database were screened using BLAST for molting, and then
the selected probes (13,504 genes) were prepared for microarray plate consisting of 60mer
oligonucleotide. After a series of processing on microarray, the resultant images were scanned
and quantified. The data were calibrated by using LOWESS nomalization, and the three
data were compared one another for gene expression pattern in blue crab during molting
stages. The result showed that increased expressed genes were chitin binding peritrophin-a
domain, antibicrobial peptide hyastatin, and arasin-like protein in the softened individuals,
while decreased expressed genes were c-type lectin domain family 2 member a-like, prophenoloxidase
activating factor, and hemocyte transglutaminase. Moreover, it was confirmed
of gene expression changes on cuticle constructing protein depending on the hardness, and
immune system activation during molting stages.