Phase Contrast Microradiography of Mouse Lung Using Synchrotron X-ray: Correlation with Optical Microscopy

다국어 초록 (Multilingual Abstract)

Purpose: The purpose of this study is to evaluate the feasibility of phase contrast X-ray microtomography and
microradiography, using a polychromatic synchrotron X-ray, for analysis of the mouse lung microstructure.
Materials and Methods: Normal mice were used for experiments. Some of the mouse lungs were prepared by the
lung fixation-inflation method. The resulting sponge-like inflated lung samples were used for microtomography.
The remaining mouse lungs were cut into 10 um sections and were used for microradiography and optical
microscopic correlation. The experiments on mouse lung samples were performed at the 7B2 beamline of the
Pohang Light Source in Korea. Results: Phase contrast X-ray microtomography of inflated lung samples showed
individual alveolar structure on 3-D reconstruction. Phase contrast microradiographs of thin lung samples showed
microstructure of lung, such as alveoli and bronchioles, and were well correlated with optical microscopic images.
Conclusions: The results indicate that the phase contrast X-ray microtomography and microradiography using
polychromatic synchrotron X-ray is feasible for evaluation of microstructure of the lung.

다국어 초록 (Multilingual Abstract)

Purpose: The purpose of this study is to evaluate the feasibility of phase contrast X-ray microtomography and
microradiography, using a polychromatic synchrotron X-ray, for analysis of the mouse lung microstructure.
Materials and Methods: Normal mice were used for experiments. Some of the mouse lungs were prepared by the
lung fixation-inflation method. The resulting sponge-like inflated lung samples were used for microtomography.
The remaining mouse lungs were cut into 10 um sections and were used for microradiography and optical
microscopic correlation. The experiments on mouse lung samples were performed at the 7B2 beamline of the
Pohang Light Source in Korea. Results: Phase contrast X-ray microtomography of inflated lung samples showed
individual alveolar structure on 3-D reconstruction. Phase contrast microradiographs of thin lung samples showed
microstructure of lung, such as alveoli and bronchioles, and were well correlated with optical microscopic images.
Conclusions: The results indicate that the phase contrast X-ray microtomography and microradiography using
polychromatic synchrotron X-ray is feasible for evaluation of microstructure of the lung.

참고문헌 (Reference)

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