Phenotypic Characterization of Ligamentum Flavum Cells from Patients with Ossification of Ligamentum Flavum

다국어 초록 (Multilingual Abstract)

Purpose: The objective of this study was to determine the phenotypic characterization of ligamentum flavum cells
from patients with ossification of the ligamentum flavum (OLF). Materials and Methods: Ligamentum flavum
tissues were harvested from OLF and non-OLF patients during surgery. OLF and non-OLF cells were isolated from
explant cultures. Cultured cells were analyzed using immunofluorescence staining and reverse transcriptionpolymerase
chain reaction. Results: OLF cells exhibited various appearances compared with the typical fibroblastlike
morphology of non-OLF cells. Expressions of collagen type I and collagen type III were observed in OLF and
non-OLF cells. OLF cells uniquely expressed osteocalcin, which is a marker for osteoblasts, and collagen type II
which is a marker for chondrocytes, whereas they were negative in non-OLF cells. Conclusion: These findings
indicate that OLF cells have phenotypic characterization of osteoblasts and chondrocytes which could play a role in
the pathophysiology of OLF.

다국어 초록 (Multilingual Abstract)

Purpose: The objective of this study was to determine the phenotypic characterization of ligamentum flavum cells
from patients with ossification of the ligamentum flavum (OLF). Materials and Methods: Ligamentum flavum
tissues were harvested from OLF and non-OLF patients during surgery. OLF and non-OLF cells were isolated from
explant cultures. Cultured cells were analyzed using immunofluorescence staining and reverse transcriptionpolymerase
chain reaction. Results: OLF cells exhibited various appearances compared with the typical fibroblastlike
morphology of non-OLF cells. Expressions of collagen type I and collagen type III were observed in OLF and
non-OLF cells. OLF cells uniquely expressed osteocalcin, which is a marker for osteoblasts, and collagen type II
which is a marker for chondrocytes, whereas they were negative in non-OLF cells. Conclusion: These findings
indicate that OLF cells have phenotypic characterization of osteoblasts and chondrocytes which could play a role in
the pathophysiology of OLF.

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