고래회충 검출을 위한 육안검사법과 중합효소연쇄반응-제한효소절편길이다형성의 비교 = Comparison of Macroscopic Inspection and Polymerase Chain Reaction- Restriction Fragment Length Polymorphism (PCR-RFLP) for the Detection of Anisakis simplex complex

다국어 초록 (Multilingual Abstract)

This research aimed to compare the detection methods of Anisakis simplex in Sea fish by polymerase
chain reaction-restriction fragment length polymorphism (PCR-RFLP) and macroscopic inspection. We examined
18 Trichiurus lepturus, 11 Scomber japonicus, and 65 Todarodes pacificus collected from the retail markets in the
areas of Uljin, Kyuonggi province and Seoul. As the result of examinations, we found that detection rate of Anisakis
simplex by macroscopic observation was 89% in Trichiurus lepturus, 90.9% in Scomber japonicus, 32.3% in Todarodes
pacificus. The detection rate of Anisakis simplex by PCR-RFLP was 77.7% in Trichiurus lepturus, 81.8% in
Scomber japonicus, 26.1% in Todarodes pacificus. We could conclude that PCR-RFLP method of Anisakis simplex
was more specific rather than macroscopic observation.

다국어 초록 (Multilingual Abstract)

This research aimed to compare the detection methods of Anisakis simplex in Sea fish by polymerase
chain reaction-restriction fragment length polymorphism (PCR-RFLP) and macroscopic inspection. We examined
18 Trichiurus lepturus, 11 Scomber japonicus, and 65 Todarodes pacificus collected from the retail markets in the
areas of Uljin, Kyuonggi province and Seoul. As the result of examinations, we found that detection rate of Anisakis
simplex by macroscopic observation was 89% in Trichiurus lepturus, 90.9% in Scomber japonicus, 32.3% in Todarodes
pacificus. The detection rate of Anisakis simplex by PCR-RFLP was 77.7% in Trichiurus lepturus, 81.8% in
Scomber japonicus, 26.1% in Todarodes pacificus. We could conclude that PCR-RFLP method of Anisakis simplex
was more specific rather than macroscopic observation.

참고문헌 (Reference)

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