표면시험법을 이용한 식품접촉표면 재질에 따른 살균소독제의 유효성 평가 = Evaluation of the Efficacy of Sanitizers on Food Contact Surfaces Using a Surface Test Method

다국어 초록 (Multilingual Abstract)

The study was undertaken to provide information on the efficacy of sanitizers against bacteria with
and without organic road dried on to food contact surfaces using the surface test method which EU and USA are currently
implementing as one of their official test methods. Escherochia coli ATCC 10536 or Staphylococcus aureus
ATCC 6538 was inoculated on to food contact surfaces, such as stainless steel, polypropylene, and silicon, which was
then treated with benzalkonium chloride, sodium hypochlorite, or ethanol as a sanitizer for 5minutes at 20℃. Results
indicated that the type of surface had little affected the efficacy of various sanitizers. In addition, 200 ppm of benzalkonium
chloride or 200 ppm of sodium hypochlorite showed no definite reduction of bacterial populations in the
present of organic load, while 40% ethanol showed reduction to 4 cfu log10/carrier or more in viable count in the
organic load.

다국어 초록 (Multilingual Abstract)

The study was undertaken to provide information on the efficacy of sanitizers against bacteria with
and without organic road dried on to food contact surfaces using the surface test method which EU and USA are currently
implementing as one of their official test methods. Escherochia coli ATCC 10536 or Staphylococcus aureus
ATCC 6538 was inoculated on to food contact surfaces, such as stainless steel, polypropylene, and silicon, which was
then treated with benzalkonium chloride, sodium hypochlorite, or ethanol as a sanitizer for 5minutes at 20℃. Results
indicated that the type of surface had little affected the efficacy of various sanitizers. In addition, 200 ppm of benzalkonium
chloride or 200 ppm of sodium hypochlorite showed no definite reduction of bacterial populations in the
present of organic load, while 40% ethanol showed reduction to 4 cfu log10/carrier or more in viable count in the
organic load.

참고문헌 (Reference)

1 김형일, "유통식품에서 분리한 Staphylococcus aureus 및Escherichia coli의 살균소독제 감수성" 한국식품과학회 39 (39): 104-107, 2007

2 김형일, "살균소독력 시험법 확립 및 살균소독력 평가" 한국식품과학회 37 (37): 838-843, 2005

3 김일진, "Vibrio parahaemolyticus 표준 및 식품분리 균주에 대한살균소독제 유효성분별 감수성 평가" 한국식품위생안전성학회 22 (22): 127-131, 2007

4 Kusumaningrum, H.D., "Survival of foodborne pathogens on stainless steel surfaces and cross-contamination to foods" 85 : 227-236, 2003

5 Gibson, H., "Surface and suspension testing: Conflict or complementary" 36 : 375-384, 1995

6 American Society for Testing and Materials, "Standard quantitative disk carrier test method for determining the bactericidal, virucidal, fungicidal, mycobactericidal and sporicidal activities of liquid chemical germicides(Designation : E 2197-02)" ASTM International 2002

7 American Society for Testing and Materials, "Standard quantitative carrier test method to evaluate the bactericidal, fungicidal, mycobactericidal and sporicidal potencies of liquid chemical germicides(Designation : E 2111-00)" ASTM International 2001

8 Oulahal, N., "Quantitative analysis of survival of Staphylococcus aureus or Listeria innocua on two types of surfaces: Polypropylene and stainless steel in contact with three different dairy products" 19 : 178-185, 2008

9 김형일, "Listeria monocytogenes와 Salmonella spp.에 대한Sodium Hypochlorite 및 Benzalkonium Chloride의 유효성 평가" 한국식품위생안전성학회 22 (22): 132-136, 2007

10 Peng, J.S., "Inactivation and removal of Bacillus cereus by sanitizer and detergent" 77 : 11-18, 2002

11 Lopes, J.A., "Evaluation of dairy and food plant sanitizers against Salmonella typhimurium and Listeria monocytogenes" 69 : 2791-2796, 1986

12 Frank, J.F., "Effectiveness of sanitation with quaternary ammonium compound or chlorine on stainless steel and other domestic food-preparation surfaces" 60 (60): 43-47, 1997

13 Fuster-Valls, N., "Effect of different environmental condotion on the bacteria survival on stainless steel surfaces" 19 : 308-314, 2008

14 Klingeren, B.V., "Disinfectant testing on surfaces" 30 : 397-408, 1995

15 Bloomfield, S., "Chlorine and iodine formulations. In Handbook of disinfectants and antiseptics" Marcel Dekker 133-158, 1996

16 European committee for standardization, "Chemical disinfectants and antiseptics-Quantitative nonporous surface test of bactericidal and/or fungicidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas-Test method and requirements(phase 2/step 2)" British standards institution 2001

17 Ronner, A.B., "Biofilm development and sanitizer inactivation of Listeria monocytogenes and Salmonella typhimurium on stainless steel and Buna-n rubber" 56 (56): 750-758, 1993

18 Mafu, A.A., "Attachment of Listeria monocytogenes to stainless steel, glass, polypropylene, and rubber surfaces after short contact times" 53 (53): 742-746, 1990

19 Bloomfield, S.F., "An evaluation of the repeatability and reproducibility of a surface test for the activity of disinfectants" 76 : 86-94, 1994