Sample pretreatment process such as concentration and separation is necessary for rapid and accurate bacteria detection. Immunomagnetic separation is one of the most widely used methods for sample pretreatment by controlling the behavior of the partic...
Sample pretreatment process such as concentration and separation is necessary for rapid and accurate bacteria detection. Immunomagnetic separation is one of the most widely used methods for sample pretreatment by controlling the behavior of the particles. However, free magnetic particles that are not bound to the bacteria can cause an error in the detection signal. In this study, the bacteria conjugated with magnetic were separated from free magnetic particles by using dielectrophoresis. The electrodes were patterned on a glass substrate with 45 degrees to the flow direction of the fluid. When an AC signal is applied to the electrodes, only bacteria are move along the electrode and flow to outlet1. On the other hand, magnetic particles flow to outlet2. The samples with a concentration of 1×10² – 1×10<SUP>6</SUP> CFU/mL were injected into the separation device and it is confirmed that the separation efficiency was greater than 80% for all samples.