Coscinoderma sp.의 대장암세포 증식 억제 효과 = Anti-proliferation Effect of Coscinoderma sp. Extract on Human Colon Cancer Cells

다국어 초록 (Multilingual Abstract)

Natural products are attractive as the source of new drug development. Especially, numerous unknown marine bioresources are an object of attention because the ocean occupies three fourth of the earth. Survival of marine bioresources in extreme environment may induce the production of biological active compounds. As previous study, we examined over 40 specimens of marine sponges collected from Micronesia and screened their anti-proliferative activities in various cancer cell lines. Among them, we investigated Coscinoderma sp.’s activity and mechanism in human colon carcinoma HCT116 and RKO cells. Furthermore, we also used the p53-knockout of HCT116 cells and the p53 loss of RKO cells for elucidating the role of p53. Coscinodermasp. inhibited cellular viability independently of the p53 status. Therefore, we compared the expression level of cell death-related proteins by Coscinodermasp. in HCT16 and in HCT116 p53KO cells. Coscinodermasp. increased p53 level and NOXA levels and induced apoptosis under the condition of p53 existence. On the other hand, Coscinodermasp. increased p21 and mTOR levels in HCT116 p53KO cells. These results suggest that Coscinodermasp. induced anti-proliferation effect through different pathway depending on p53 status.

참고문헌 (Reference)

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