소목의 열수 및 에탄올 추출물의 항산화 및 항암활성

다국어 초록 (Multilingual Abstract)

Caesalpinia sappan L. is an oriental medicinal plant distributed in the Asia Pacific region including India, Malaysia, and China. The dried heartwood of Caesalpinia sappan has been traditionally used as an analgesic and anti-inflammatory drug. In this study, the effects of extract methods of C. sappan on contents of total polyphenols and flavonoids, antioxidant activity, and cytotoxic activity were evaluated. As a result, hot water extract from C. sappan (CSWE) significantly exhibited contents of total polyphenols and flavonoids (22.6 mg GAE/g and 14.5 mg QE/g) higher than 70% ethanol extract (CSEE) (17.6 mg GAE/g and 13.2 mg QE/g). However, CSEE showed greater antioxidant activity than CSWE in both DPPH and ABTS. Also, the cytotoxicity of C. sappan against three kinds of cancer cell lines was higher in CSEE than in CSWE. These results show that ethanol extract is a better extract method than hot water method to maintain antioxidant and anti-cancer activities.

참고문헌 (Reference)

1 곽충실, "한국산 메밀, 수수, 기장, 율무의 항산화효과 및 돌연변이억제효과" 한국식품영양과학회 33 (33): 921-929, 2004

2 한만덕, "인체 상피세포주와 암세포주에 대한 소목 추출물의 증식억제 효과" 한국치위생과학회 7 (7): 31-35, 2007

3 임현지, "아사이베리, 블루베리, 산수유, 오디 에탄올 추출물의 항산화 활성도비교 분석" 한국피부과학연구원 13 (13): 445-452, 2015

4 최선영, "식용 및 약용자원의 에스트로젠 활성과 항산화능 평가" 한국식품과학회 37 (37): 549-556, 2005

5 이승은, "시판 한약재에 대한 항산화 활성 검정" 한국약용작물학회 10 (10): 171-176, 2002

6 전미애, "소목(Caesalpinia sappan L.)에서 분리한 Brazilin의 항산화 활성과 구강상피암 KB 세포주의 증식억제효과" 한국치위생과학회 10 (10): 365-372, 2010

7 이종수, "소목 추출물의 구강암 및 골육종 세포주에 대한 항암작용에 관한 연구" 대한구강악안면외과학회 33 (33): 583-590, 2007

8 전연희, "복분자 딸기(Rubus coreanum) 에탄올 및 열수추출물의 항돌연변이 활성과 암세포 성장 억제 효과" 한국식품조리과학회 25 (25): 379-386, 2009

9 김유나, "고추씨 물추출물이 고지방과 고콜레스테롤 식이 흰쥐의 항산화 효소활성 및 산화적 손상에 미치는 영향" 한국영양학회 44 (44): 284-291, 2011

10 강성순, "蘇木이 산화적 손상 및 Raw 264.7 cell과 HUVEC에서의 염증 유관 인자에 미치는 영향" 대한한방내과학회 34 (34): 100-111, 2013

11 Xie YW, "Vasorelaxing effects of Caesalpinia sappan involvement of endogenous nitric oxide" 67 : 1913-1918, 2000

12 Halliwell B, "The importance of free radicals and catalytic metal ions in human diseases" 8 : 89-193, 1985

13 Doll R, "The causes of cancer : Quantitive estimates of avoiable risks of cancer in the United States today" 66 : 1191-1308, 1981

14 Alessio HM., "Oxygen-radical absorbance capacity assay for antioxidants" 14 : 303-311, 1993

15 Folin O, "On phosphotungstic-phosphomolybdic compounds as color reagents" 7 : 239-243, 1912

16 Wink D, "Nitric oxide protects against cellular damage and cytotoxicity from reactive oxygen species" 90 : 9813-9817, 1993

17 Chang MC, "Induction of necrosis and apoptosis to KB cancer cells by sanguinarine is associated with reactive oxygen species production and mitochondrial membrane depolarization" 218 : 143-151, 2007

18 Kim JK., "Illustrated Natural Drugs Encyclopedia" Namsandang Publishers 414-415, 1989

19 Srilakshmi VS, "Hepato protective properties of Caesalpinia sappan Linn. heartwood on carbontetra chloride induced toxicity" 48 : 905-910, 2010

20 Wang YZ, "Extract of the dried heartwoodof Caesalpinia sappan L. attenuates collagen-induced arthritis" 136 : 271-278, 2011

21 Jenkins R., "Exercise, oxidative stress, and antioxidants: A review" 3 : 356-375, 1993

22 Carmichael J, "Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing" 47 : 936-942, 1987

23 Moreno MI, "Comparison of the free radical-scavenging activity of propolis several regions of Argentina" 71 : 109-114, 2000

24 Banasiak D, "Comparison between the clonogenic, MTT, and SRB assays for determining radiosensitivity in a panel of human bladder cancer cell lines and a ureteral cell line" 7 : 77-85, 1999

25 Wang Z, "Caesappin A and B, two novel protosappanins from Caesalpinia sappn L" 92 : 280-284, 2014

26 Toegel S, "Caesalpinina sappan extract inhibits IL 1bmediated overexpression of matrix metalloproteinases in human chondrocytes" 7 : 312-318, 2012

27 Badami S, "Antioxidantactivity of Caesalpinia sappan heartwood" 26 : 1534-1537, 2003

28 Lee KS., "Antioxidant, antimicrobial effect of the extracts of cactus cheonnyuncho (Opuntia humifusa) and identification of activity substance" Hoseo Univ. 2005

29 Blios MS, "Antioxidant determination by the use of a stable free radical" 181 : 1199-1200, 1958

30 Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay" 26 : 1231-1237, 1999

31 Tewtrakul S, "Antiinflammatory and wound healing effects of Caesalpinia sappan L" 29 : 850-856, 2015

32 Baek NI, "Anticonvulsant compounds from the wood of Caesalpinia sappan L" 23 : 344-348, 2000

33 Wu SQ, "Anti-inflammatory activity of an ethanolic Caesalpinia sappan extract in human chondrocytes and macrophages" 138 : 364-372, 2011