Chaperone function of Arabidopsis NPR1

다국어 초록 (Multilingual Abstract)

To cope with diverse environmental stresses, the sessile organisms of plants have evolved diverse defense molecules and signaling systems to transfer the stress signals into downstream metabolic cascades and induce the expression of defenseassociated genes. Among the defense systems, the Arabidopsis nonexpressor of pathogenesis-related protein 1 (AtNPR1) playing a key role in a plant systemic acquired immune responses has been shown to have multiple functions. The molecular structure of AtNPR1 has two domains, BTB/POZ and ANK repeat, that are involved in protein–protein interactions. Despite the function of its salicylic acid-induced defense activity in nucleus, the biochemical property of its cytosolic oligomers has not been elucidated. Based on the results that the reversible structural change of redox proteins is a typical property of molecular chaperones, we investigated the biochemical characteristics of AtNPR1 after expressing it in E. coli and purifying the protein. From the study, the recombinant AtNPR1 functions as a protein chaperone to protect plants from heat stress through its structural switching by its oligomer form. Under heat-induced (43 °C) condition, the AtNPR1 protein prevents from aggregation of substrate, MDH. And the structural change was regulated upon the redox changes, such as DTT treatment dissociated its structure to monomer and reduced its chaperone activity, suggesting that the heat-induced chaperone activity of AtNPR1 is dependent on its redox status. In summary, the cytosolic AtNPR1 oligomer performs the important function of molecular chaperone to protect plants from heat stress that can be applied to the preparation of heat shock-tolerant useful crops.

참고문헌 (Reference)

1 Jang HH, "Two enzymes in one; two yeast peroxiredoxins display oxidative stress-dependent switching from a peroxidase to a molecular chaperone function" 117 : 625-635, 2004

2 Chae HB, "Thioredoxin Reductase Type C (ntrc) orchestrates enhanced thermotolerance to Arabidopsis by its redox-dependent holdase chaperone function" 6 : 323-336, 2013

3 Collet J-F, "Thioredoxin 2, an oxidative stress-induced protein, contains a high affinity zinc binding site" 278 : 45325-45332, 2003

4 Brodersen P, "The role of salicylic acid in the induction of cell death in Arabidopsis acd11" 138 : 1037-1045, 2005

5 Mosavi LK, "The ankyrin repeat as molecular architecture for protein recognition" 13 : 1435-1448, 2004

6 Sedgwick SG, "The ankyrin repeat : a diversity of interactions on a common structural framework" 24 : 311-316, 1999

7 Bardwell VJ, "The POZ domain : a conserved protein-protein interaction motif" 8 : 1664-1677, 1994

8 Albagli O, "The BTB/POZ domain:a new protein-protein interaction motif common to DNA- and actin-binding proteins" 6 : 1193-1198, 1995

9 Cao H, "The Arabidopsis NPR1gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats" 88 : 57-63, 1997

10 Wu Y, "The Arabidopsis NPR1 protein is a receptor for the plant defense hormone salicylic acid" 1 : 639-647, 2012

11 Weis F, "The 90-kDa heat shock protein Hsp90 protects tubulin against thermal denaturation" 285 : 9525-9534, 2010

12 Lee H-J, "Systemic immunity requires snrk2.8-mediated nuclear import of npr1 in Arabidopsis" 27 : 3425-3438, 2015

13 Conrath U, "Systemic acquired resistance" 1 : 179-184, 2006

14 Durrant WE, "Systemic acquired resistance" 42 : 185-209, 2004

15 Lee EM, "Site-directed mutagenesis substituting cysteine for serine in 2-Cys peroxiredoxin (2-Cys Prx A) of Arabidopsis thaliana effectively improves its peroxidase and chaperone functions" 116 : 713-725, 2015

16 Chi YH, "Redox-dependent functional switching of plant proteins accompanying with their structural changes" 4 : 277-, 2013

17 Zhou M, "Redox rhythm reinforces the circadian clock to gate immune response" 523 : 472-476, 2015

18 Choudhury FK, "Reactive oxygen species, abiotic stress and stress combination" 90 : 856-867, 2017

19 Saijo Y, "Plant immunity in signal integration between biotic and abiotic stress responses" 225 : 87-104, 2020

20 Verma V, "Plant hormone-mediated regulation of stress responses" 16 : 86-, 2016

21 Ku Y-S, "Plant hormone signaling crosstalks between biotic and abiotic stress responses" 19 : 3206-, 2018

22 Jang HH, "Phosphorylation and concomitant structural changes in human 2-Cys peroxiredoxin isotype I differentially regulate its peroxidase and molecular chaperone functions" 580 : 351-355, 2006

23 Wood ZA, "Peroxiredoxin evolution and the regulation of hydrogen peroxide signaling" 300 : 650-653, 2003

24 Malnoy M, "Overexpression of the apple MpNPR1 gene confers increased disease resistance in malus × domestica" 20 : 1568-1580, 2007

25 Olate E, "NPR1mediates a novel regulatory pathway in cold acclimation by interacting with HSFA1 factors" 4 : 811-823, 2018

26 Dong X, "NPR1, all things considered" 7 : 547-552, 2004

27 Silva KJP, "NPR1 as a transgenic crop protection strategy in horticultural species" 5 : 15-, 2018

28 Zhang J, "NPR1 and redox rhythm : connections, between circadian clock and plant immunity" 20 : 1211-, 2019

29 Huang H, "Mechanisms of ROS regulation of plant development and stress responses" 10 : 800-, 2019

30 Zhang Y, "Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene" 96 : 6523-6528, 1999

31 Sharma KK, "Interaction of 1,1′-Bi(4-anilino)naphthalene-5,5′-disulfonic acid with a-crystallin" 273 : 8965-8970, 1998

32 Mou Z, "Inducers of plant systemic acquired resistance regulate NPR1 function through redox changes" 113 : 935-944, 2003

33 Heath MC, "Hypersensitive response-related death" 44 : 321-334, 2000

34 Mozo-Villarías A, "Hydrophobicity density profi les to predict thermal stability enhancement in proteins" 25 : 529-535, 2006

35 Lee JR, "Heat-shock dependent oligomeric status alters the function of a plant-specific thioredoxin-like protein, AtTDX" 106 : 5978-5983, 2009

36 Park SK, "Heat-shock and redox-dependent functional switching of an h-type Arabidopsis thioredoxin from a disulfi de reductase to a molecular chaperone" 150 : 552-561, 2009

37 Yonehara M, "Heat-induced chaperone activity of HSP90" 271 : 2641-2645, 1996

38 Cao H, "Generation of broad-spectrum disease resistance by overexpression of an essential regulatory gene in systemic acquired resistance" 95 : 6531-6536, 1998

39 Voronin DA, "Functional role of proteins containing ankyrin repeats" 2 : 1-12, 2008

40 Pessler F, "Flexible DNA binding of the BTB/POZ-domain protein FBI-1" 278 : 29327-29335, 2003

41 Sharma M, "Expansion and Function of repeat domain proteins during stress and development in plants" 6 : 1218-, 2016

42 Chern MS, "Evidence for a disease-resistance pathway in rice similar to the NPR1-mediated signaling pathway in Arabidopsis" 27 : 101-113, 2001

43 Suzuki N, "Enhanced seed production under prolonged heat stress conditions in Arabidopsis thaliana plants defi cient in cytosolic ascorbate peroxidase 2" 64 : 253-263, 2013

44 Suzuki N, "Coordination between ros regulatory systems and other pathways under heat stress and pathogen attack" 9 : 490-, 2018

45 Miranda-Vizuete A, "Cloning, expression, and characterization of a novel Escherichia coli thioredoxin" 272 : 30841-30847, 1997

46 Reddy GB, "Chaperone-like activity and hydrophobicity of alpha-crystallin" 58 : 632-641, 2006

47 Jakob U, "Chaperone activity with a redox switch" 96 : 341-352, 1999

48 Cohen SP, "Abiotic and biotic stresses induce a core transcriptome response in rice" 9 : 1-11, 2019