PCR 특이검출에 의한 국내 포도나무 혹병(Agrobacterium vitis) 균주의 신속 분리 및 병원학적, 생화학적 특성 비교

다국어 초록 (Multilingual Abstract)

Agrobacterium vitis is a causal agent of crown-gall disease on grapevine. Bacterial strains were isolated from
crown galls of different grapevine varieties in grapevine farms of Kyungbuk (Kimcheon), Chungbuk
(Okcheon), Chungnam (Daejon, Choenan) and Kyeonggi (Suwon, Ansung) areas in Korea from 2002 to 2005.
Primer sets, Phe A and VirA, which were derived from pectate lysase gene and virA gene of Ti-plasmid in A.
vitis were used to detect A. vitis strains from crown galls. PheA and VirA primers amplified DNA fragments of
0.25 kb and 0.5 kb from fifty-one bacterialstrains. They formed crown galls on grapevine variety, Kyoho, or
carrot disks with variable pathgenecity It was confirmed that the biochemical characteristics of 10 bacterial
strains that was strong pathogenecity on grapevine were mostly in agreement with type culture strains of A.
vitis, showing growth in the presence of 2% NaCl, non-production of acid from melezitose and negative
response in production of 3-Ketolactose.

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